Keratin, type II cytoskeletal 1b (KRT77)
The protein contains 578 amino acids for an estimated molecular weight of 61901 Da.
No function (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs17118224 |
No binding partner found
The reference OMIM entry for this protein is 611158
Keratin 77, type ii; krt77
K77
Kb39
Keratin 1b; k1b
DESCRIPTION
Keratins, such as KRT77, are filament proteins that make up one of the major structural fibers of epithelial cells (Rogers et al., 2005).CLONING
Using database analysis to identify keratin genes within the type II keratin gene domain on chromosome 12, Rogers et al. (2005) identified KRT77, which they called K1B. The deduced 578-amino acid protein has a calculated molecular mass of 61.8 kD. Like other type II keratins, K1B has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences. In addition, it has numerous GGG and GGX repeats in its head and tail domains. Northern blot analysis detected weak expression of a 3.0-kb K1B transcript in skin, but not in scalp or in any other tissues or organs examined. Using in situ hybridization and indirect immunofluorescence, Langbein et al. (2005) detected strong K1B expression in the ducts of eccrine sweat glands, but not in the deeper secretory portion of the glands.MAPPING
By genomic sequence analysis, Rogers et al. (2005) mapped the KRT77 gene to chromosome 12q13.13. ... More on the omim web site
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 611158 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).