Nuclear receptor 2C2-associated protein (NR2C2AP)
The protein contains 139 amino acids for an estimated molecular weight of 15876 Da.
May act as a repressor of NR2C2-mediated transactivation by suppressing the binding between NR2C2/TR4 and the TR4-response element in target genes. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
Molecular Function
The reference OMIM entry for this protein is 608719
Tr4-associated protein, 16-kd
Tra16
CLONING
Using TR4 (601426) in a yeast 2-hybrid screen of a testis cDNA library, followed by 5-prime RACE, Yang et al. (2003) cloned TRA16. The deduced 139-amino acid protein has a calculated molecular mass of 16 kD. Northern blot analysis detected a transcript of about 1.0 kb in all tissues examined, with highest expression in heart, skeletal muscle, and pancreas. Immunofluorescence microscopy detected endogenous TRA16 predominantly in nuclei of COS-1 cells and in several human cell lines.GENE FUNCTION
By coimmunoprecipitation of a transfected nonsmall cell lung carcinoma cell line, Yang et al. (2003) confirmed interaction between TRA16 and TR4. Electrophoretic mobility shift assay suggested that TRA16 may suppress binding between TR4 and the TR4 response element in target genes. Yang et al. (2003) also presented evidence that TRA16 blocks TR4 dimerization.MAPPING
By genomic sequence analysis, Yang et al. (2003) mapped the TRA16 gene to chromosome 19p12. ... More on the omim web site
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 608719 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).