Zinc transporter 7 (SLC30A7)
The protein contains 376 amino acids for an estimated molecular weight of 41626 Da.
Seems to facilitate zinc transport from the cytoplasm into the Golgi apparatus. Partly regulates cellular zinc homeostasis. Required with ZNT5 for the activation of zinc-requiring enzymes, alkaline phosphatases (ALPs). Transports zinc into the lumens of the Golgi apparatus and the vesicular compartments where ALPs locate, thus, converting apoALPs to holoALPs. Required with ZNT5 and ZNT6 for the activation of TNAP (By similarity). (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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No binding partner found
Biological Process
Regulation of sequestering of zinc ion
Response to zinc ion
Sequestering of zinc ion
Zinc ion transport
The reference OMIM entry for this protein is 611149
Solute carrier family 30 (zinc transporter), member 7; slc30a7
Zinc transporter 7; znt7
DESCRIPTION
Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A7, permit cellular efflux of zinc (Seve et al., 2004).CLONING
Kirschke and Huang (2003) identified mouse and human SLC30A7, which they called ZNT7. The deduced proteins share 96% amino acid identity. Mouse Znt7 contains 6 transmembrane domains, a histidine-rich loop between transmembrane domains 4 and 5, cytoplasmic N and C termini, several phosphorylation sites, and an N-glycosylation site. Northern blot analysis of mouse tissues detected highest expression in liver, spleen, and small intestine. In rat epithelial and human fibroblast cell lines, ZNT7 clustered at the perinuclear region and showed punctate staining in the cytoplasm, indicative of cytoplasmic vesicle localization. By EST database analysis, Seve et al. (2004) found evidence of moderate SLC30A7 expression in colon and eye, with lower levels in bone marrow, breast, liver, prostate, skin, and T cells.GENE FUNCTION
Kirschke and Huang (2003) found that Chinese hamster ovary cells expressing mouse Znt7 accumulated zinc from the culture medium into the Golgi apparatus. Using chicken B cells lacking Znt5 (607819), Znt6 (611148), and Znt7, followed by reexpression of the human transgenes, Suzuki et al. (2005) showed that ZNT5 and ZNT6 had to be coexpressed to transport zinc for activation of tissue-nonspecific alkaline phosphatase (TNAP, or ALPL; 171760) in the Golgi lumen, whereas ZNT7 acted alone to perform the same function. Coimmunoprecipitation and immunofluorescence analyses showed that ZNT5 and ZNT6 formed heterooligomers and that ZNT7 formed homooligomers.GENE STRUCTURE
Kirschke and Huang (2003) determined that the SLC30A7 gene contains at least 11 exons.MAPPING
By genomic sequence analysis, Kirschke and Huang (2003) mapped the SLC30A7 gene to chromosome 1p13.3. They mapped the mouse Slc30a7 gene to a region of chromosome 3 that shares homology of synteny with human chromosome 1p13.3. Seve et al. (2004) stated that the SLC30A7 gene maps to chromosome 1p21.2. ... More on the omim web site
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 611149 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).