Choline transporter-like protein 1 (SLC44A1)
The protein contains 657 amino acids for an estimated molecular weight of 73302 Da.
Choline transporter. Involved in membrane synthesis and myelin production. (updated: April 7, 2021)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs3199966 |
No binding partner found
Biological Process
Choline catabolic process
Choline transport
Phosphatidylcholine biosynthetic process
Transmembrane transport
Transport across blood-brain barrier
The reference OMIM entry for this protein is 606105
Solute carrier family 44, member 1; slc44a1
Choline transporter-like protein 1; ctl1
Cdw92
CLONING
Choline, a major constituent of cell membranes, participates in the synthesis of acetylcholine in cholinergic neurons. It is taken up with high affinity at cholinergic nerve terminals in a sodium-dependent manner. O'Regan et al. (2000) cloned CTL1, a suppressor for a yeast choline transport mutation, from a Torpedo electric lobe yeast expression library by functional complementation. By EST database searching and PCR on a sarcoma cell line, they obtained cDNAs encoding human CTL1, CTL2 (606106), and CTL4 (606107). Sequence analysis predicted that the 654-amino acid CTL1 protein is 96% identical to the rat Ctl1 protein and 43% homologous to human CTL2 and CTL4. The CTL proteins contain 10 transmembrane domains, 11 highly conserved cysteines, and no signal peptide. Northern blot analysis of rat tissue revealed expression of a 3.5-kb Ctl1 transcript in spinal cord, with lower expression in brain, while a 5.0-kb transcript was expressed in colon, with lower expression in lung and spinal cord. In situ hybridization analysis demonstrated expression of rat Ctl1 in motor neurons of the spinal cord ventral horn and in gray and white matter of the cord. Expression was also detected in myelinated fiber tracts in the brain and in cerebellar white matter, brainstem, corpus callosum, hippocampal fimbria, the lateral olfactory tract, and oligodendrocytes. Using an expression cloning approach, Wille et al. (2001) obtained a cDNA encoding CDw92. The deduced 652-amino acid CDw92 protein is 99% identical to the CTL1 protein reported by O'Regan et al. (2000). CDw92 has cytoplasmic N and C termini, 3 potential N-glycosylation sites, and a potential ITIM (immunoglobulin tyrosine-based inhibitory motif). Immunoprecipitation analysis showed that CDw92 is expressed as a 70-kD protein. Flow cytometric analysis demonstrated expression of CDw92 on almost all B lymphocytes, most T lymphocytes, some NK cells, and some myeloid cell lines tested. Maturation of monocyte/dendritic cells resulted in downregulation of CDw92, and expression could subsequently be upregulated by interleukin-10 (IL10; 124092).MAPPING
By STS and YAC analysis, O'Regan et al. (2000) mapped the CTL1 gene to 9q31.2. ... More on the omim web site
April 10, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 606105 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).