ADP-ribosylation factor-like protein 8A (ARL8A)
The protein contains 186 amino acids for an estimated molecular weight of 21416 Da.
Plays a role in lysosome motility (By similarity). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation (By similarity). (updated: Feb. 13, 2019)
Protein identification was indicated in the following studies:
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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No binding partner found
Biological Process
Anterograde axonal transport
Cell cycle
Cell division
Chromosome segregation
Neutrophil degranulation
Protein transport
The reference OMIM entry for this protein is 616597
Adp-ribosylation factor-like 8a; arl8a
Small g protein indispensable for equal chromosome segregation 2; gie2
DESCRIPTION
Small GTPases function as molecular switches and work in conjunction with a range of cellular targets to elicit or regulate biologic function. ARL8A and ARL8B (616596) form a distinct subfamily of small GTPases (Okai et al., 2004).CLONING
By database analysis to identify novel small GTPases, followed by PCR of a human brain cDNA library, Okai et al. (2004) cloned ARL8A, which they called GIE2. The deduced 186-amino acid GIE2 protein has 5 motifs conserved in small GTPases, including GTP-binding domains and a putative effector domain, but it lacks a putative lipid modification motif found in other small GTPases. GIE2 shares 91% identity with GIE1 (ARL8B), and the GIE proteins share about 30% identity with other small GTPases. Northern blot analysis of 12 human tissues detected ubiquitous expression of a 2.0-kb GIE2 transcript. An antibody that did not differentiate between GIE1 and GIE2 detected an apparent 22-kD polypeptide in rat PC12 cells. Immunohistochemical analysis revealed that GIE proteins localized to the cytoplasm along with microtubules during interphase, then redistributed to spindle midzone in anaphase and to the midbody in late telophase. Database analysis revealed 2 GIE genes in mammals, a single GIE ortholog in Drosophila and C. elegans, and no ortholog in yeast.GENE FUNCTION
Using thin-layer chromatography, Okai et al. (2004) found both GTP- and GDP-bound forms of epitope-tagged GIE2 following expression in HeLa cells. They concluded that GIE2 cycles between both nucleotide-bound forms.MAPPING
By genomic sequence analysis, Okai et al. (2004) mapped the ARL8A gene to chromosome 1q32.1. ... More on the omim web site
Feb. 22, 2019: Protein entry updated
Automatic update: comparative model was added.
Feb. 22, 2019: Protein entry updated
Automatic update: model status changed
Feb. 22, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).
Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 616597 was added.