Protein Niban 2 (FAM129B)

The protein contains 746 amino acids for an estimated molecular weight of 84138 Da.

 

May play a role in apoptosis suppression. May promote melanoma cell invasion in vitro. (updated: July 31, 2019)

Protein identification was indicated in the following studies:

  1. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  2. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  3. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology.


Interpro domains
Total structural coverage: 0%
Model score: 0
No model available.

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The reference OMIM entry for this protein is 614045

Family with sequence similarity 129, member b; fam129b
Melanoma invasion by erk; minerva

CLONING

Chen et al. (2011) cloned human FAM129B. The deduced 746-amino acid protein has a calculated molecular mass of 83 kD. It contains an N-terminal pleckstrin (PLEK; 173570) homology (PH) domain, a C-terminal proline-rich domain, and 6 potential serine phosphorylation sites. In growing HeLa cells and mouse embryonic fibroblasts, FAM129B localized to the cytoplasmic compartment. In confluent HeLa cells, FAM129B relocalized to the plasma membrane near cell junctions.

GENE FUNCTION

Chen et al. (2011) found that knockdown of FAM129B in HeLa cells did not itself induce apoptosis, but it accelerated the onset of apoptosis induced by TNF-alpha (TNF; 191160). Apoptosis proceeded through a caspase (see 600636)-dependent and proteasome-independent pathway, and FAM129B was degraded during apoptosis. In melanoma and HeLa cells, FAM129B localized to the cytoplasm during an active ERK1 (MAPK3; 601795)/ERK2 (MAPK1; 176948) MAP kinase cascade. FAM129B localized to cell junctions upon inhibition of the MAP kinase cascade and upon growth to confluence. FAM129B also localized to the plasma membrane during mitosis.

GENE STRUCTURE

Chen et al. (2011) determined that the FAM129B gene contains 3 exons.

MAPPING

Hartz (2011) mapped the FAM129B gene to chromosome 9q34.13 based on an alignment of the FAM129B sequence (GenBank GENBANK AL137555) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

Aug. 20, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.

Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 614045 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).