ADP-dependent glucokinase (ADPGK)
The protein contains 497 amino acids for an estimated molecular weight of 54089 Da.
Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity). (updated: June 1, 2001)
Protein identification was indicated in the following studies:
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs8024644 |
No binding partner found
Biological Process
Canonical glycolysis
Glucose metabolic process
Glycolytic process through glucose-6-phosphate
Cellular Component
Endoplasmic reticulum
Endoplasmic reticulum membrane
Extracellular region
Membrane
The reference OMIM entry for this protein is 611861
Adp-dependent glucokinase; adpgk
Adp-gk
DESCRIPTION
ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004).CLONING
By database analysis, Ronimus and Morgan (2004) identified human ADPGK. They obtained a mouse Adpgk cDNA derived from mammary tumor and determined by SDS-PAGE that Adpgk has a molecular mass of 51.5 kD. Gel filtration analysis showed that the protein likely is monomeric. Database analysis showed expression in mammalian epithelial, endocrine, lymphatic, muscular, and organ tissues, consistent with a housekeeping function for Adpgk.GENE FUNCTION
Using recombinant mouse Adpgk purified to homogeneity by SDS-PAGE, Ronimus and Morgan (2004) showed that Adpgk exhibited ADP-dependent glucokinase activity and had a bimodal optimal pH at 5.75 to 6.5 and 8.75 to 9.0. Activity was not inhibited by glucose-6-phosphate and other known modulators of glycolytic enzymes; however, high concentrations of glucose significantly inhibited enzyme activity. Mouse Adpgk displayed high specificity for D-glucose, low Km for glucose and ADP, and inhibition by glucose and AMP. Based on these characteristics, Ronimus and Morgan (2004) concluded that Adpgk is a member of the mammalian hexokinase/glucokinase family (see HK1; 142600)GENE STRUCTURE
Ronimus and Morgan (2004) determined that the ADPGK gene contains 7 exons and 4 predicted alternative promoters.MAPPING
By genomic sequence analysis, Ronimus and Morgan (2004) mapped the ADPGK gene to chromosome 15. They mapped the corresponding mouse Adpgk gene to mouse chromosome 9. ... More on the omim web site
June 29, 2020: Protein entry updated
Automatic update: OMIM entry 611861 was added.
Feb. 22, 2019: Protein entry updated
Automatic update: comparative model was added.
Feb. 22, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).