Leucine-rich repeat transmembrane protein FLRT1 (FLRT1)
The protein contains 646 amino acids for an estimated molecular weight of 71358 Da.
Plays a role in fibroblast growth factor-mediated signaling cascades that lead to the activation of MAP kinases. Promotes neurite outgrowth via FGFR1-mediated activation of downstream MAP kinases. Promotes an increase both in neurite number and in neurite length. May play a role in cell-cell adhesion and cell guidance via its interaction with ADGRL1/LPHN1 and ADGRL3. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.
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Biological Process
Cell adhesion
Dendrite development
Fibroblast growth factor receptor signaling pathway
Neuron projection extension
Positive regulation of synapse assembly
Cellular Component
Cell-cell junction
Cytoplasmic vesicle
Cytoplasmic vesicle membrane
Endoplasmic reticulum membrane
Extracellular matrix
Extracellular space
Focal adhesion
Integral component of plasma membrane
Neuron projection terminus
Neuronal cell body membrane
Perinuclear region of cytoplasm
Plasma membrane
The reference OMIM entry for this protein is 604806
Fibronectin-like domain-containing leucine-rich transmembrane protein 1; flrt1
CLONING
The extracellular matrix (ECM) is composed of collagens, proteoglycans, and noncollagenous glycoproteins that provide cells and tissues with a mechanical scaffold for adhesion, migration, and signal transduction. These varied and complex functions depend on interactions between ECM components and cellular receptors, such as integrins and proteoglycans, located at the cell surface. To identify cDNAs encoding ECM proteins that are expressed in muscle, Lacy et al. (1999) screened a human adult muscle cDNA library with a degenerate oligonucleotide that encodes a sequence conserved in the G2 domains of human nidogen-1 and -2 (see NID; 131390) and mouse enactin-1. They isolated several cDNAs, including a partial cDNA encoding FLRT1. Lacy et al. (1999) assembled a full-length FLRT1 coding sequence. The predicted 674-amino acid FLRT1 protein contains a putative N-terminal signal sequence, 10 leucine-rich repeats (LRRs) flanked by N- and C-terminal cysteine-rich regions, a fibronectin-/collagen-like domain, a transmembrane domain, and an intracellular C-terminal tail. FLRT1 has 2 potential N-glycosylation sites in its extracellular region. FLRT1 shares 55% amino acid sequence identity with FLRT3 (604808) and 41% identity with FLRT2 (604807). Recombinant FLRT1 expressed in SF9 insect cells and monkey COS-1 cells migrated as a 90-kD protein on SDS-polyacrylamide gels. The authors demonstrated that FLRT1 is glycosylated. Northern blot analysis of a variety of human adult and fetal tissues detected a major 4.4-kb FLRT1 transcript only in adult and fetal brain and kidney. Within adult brain, FLRT1 expression was found in all subregions examined, namely putamen, temporal lobe, frontal lobe, occipital lobe, medulla, cortex, cerebellum, and spinal cord; besides the 4.4-kb transcript, an additional 6-kb FLRT1 transcript was present in all subregions except cerebellum. Lacy et al. (1999) suggested that FLRT1 functions in cell adhesion and/or receptor signaling.MAPPING
By FISH, Lacy et al. (1999) mapped the FLRT1 gene to 11q12-q13. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 604806 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).