A-kinase anchor protein 7 isoform gamma (AKAP7)

The protein contains 348 amino acids for an estimated molecular weight of 39518 Da.

 

Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium. (updated: Oct. 10, 2018)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 0%
Model score: 42

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VariantDescription
dbSNP:rs7771473
dbSNP:rs1190788

No binding partner found

The reference OMIM entry for this protein is 604693

A-kinase anchor protein 7; akap7
A-kinase anchor protein, 18-kd; akap18

DESCRIPTION

A-kinase anchor proteins (AKAPs; see 602449) direct the activity of protein kinase A (PKA; see 176911) by tethering the enzyme near its physiologic substrates.

CLONING

By screening a fetal brain cDNA library with regulatory type II (RII) PKA as a probe, Fraser et al. (1998) obtained a cDNA encoding a novel AKAP protein, AKAP7, which the authors designated AKAP18. AKAP7 contains 81 amino acids. Northern blot analysis demonstrated the expression of a major 2.9-kb transcript in pancreas, brain, and heart as well as a minor band of 4.3 kb in cardiac and skeletal muscle. Helical wheel analysis showed that the RII-binding amphipathic helix domain comprises residues 29 to 42. Sequence analysis identified a myristoylation signal at the N-terminal glycine residue and 2 palmitoylation sites at cys4 and cys5. Immunoprecipitation and immunofluorescence confocal microscopy analyses of wildtype and mutant AKAP18 showed that the lipids are required for attachment of AKAP18 to the cytoplasmic face of the plasma membrane. Expression of AKAP18 in cells expressing cardiac L-type calcium channels promoted an increase in cAMP-responsive calcium currents, suggesting that membrane anchoring of PKA participates in physiologically relevant events involving ion channel activation.

MAPPING

The International Radiation Hybrid Mapping Corsortium mapped the AKAP7 gene to chromosome 6 (TMAP stSG41406). ... More on the omim web site

Subscribe to this protein entry history

Dec. 9, 2018: Protein entry updated
Automatic update: model status changed

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).

Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 604693 was added.