Translocon-associated protein subunit gamma (SSR3)
The protein contains 185 amino acids for an estimated molecular weight of 21080 Da.
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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Cellular Component
Endoplasmic reticulum
Endoplasmic reticulum membrane
Integral component of membrane
Molecular Function
The reference OMIM entry for this protein is 606213
Signal sequence receptor, gamma; ssr3
Translocon-associated protein, gamma subunit; trapg
DESCRIPTION
Proteins are translocated across the endoplasmic reticulum (ER) at specific sites called translocons, probably through protein-conducting channels. Signal sequence receptors, such as SSR3, are associated with the TRAM protein (605190), the major crosslinking partner in the translocation activity (summary by Hartmann et al., 1993).CLONING
By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein.GENE FUNCTION
Hartmann et al. (1993) found that Ssr3 was associated in equal amounts with Ssr1 (600868), Ssr2 (600867), and Ssr4 (300090) in canine pancreatic microsomes.MAPPING
Gross (2012) mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence (GenBank GENBANK AF087907) with the genomic sequence (GRCh37). ... More on the omim web site
May 13, 2019: Protein entry updated
Automatic update: OMIM entry 606213 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).