Casein kinase I isoform gamma-3 (CSNK1G3)
The protein contains 447 amino acids for an estimated molecular weight of 51389 Da.
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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The reference OMIM entry for this protein is 604253
Casein kinase i, gamma-3; csnk1g3
CLONING
The casein kinase I (CKI) gene family encodes serine/threonine kinases that preferentially phosphorylate acidic substrates using ATP as a phosphate donor. CKI proteins are monomeric and range from 25 to 55 kD. They are ubiquitous, being found in the nuclei, cytoplasm, and membrane fractions of eukaryotic cells. By screening a human testis cDNA library with a rat CKI gamma-3 cDNA, Kusuda et al. (1998) isolated a cDNA encoding CSNK1G3. The deduced 447-amino acid CSNK1G3 protein shares 98% sequence identity with rat CKI gamma-3. The authors also isolated a cDNA whose sequence is identical to that of the aforementioned CSNK1G3 cDNA except that it contains a 24-bp insertion in the coding sequence and an 8-bp insertion in the 3-prime untranslated region. They suggested that this cDNA represents an alternatively spliced CSNK1G3 transcript, which they named CSNK1G3L.MAPPING
By radiation hybrid mapping and FISH, Kusuda et al. (1998) mapped the CSNK1G3 gene to 5q23. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 604253 was added.
Feb. 22, 2019: Protein entry updated
Automatic update: comparative model was added.
Feb. 22, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).