Guided entry of tail-anchored proteins factor 1 (WRB)
The protein contains 174 amino acids for an estimated molecular weight of 19780 Da.
Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER) (PubMed:21444755, PubMed:23041287, PubMed:24392163, PubMed:27226539). Together with CAMLG/GET2, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol (PubMed:21444755, PubMed:23041287, PubMed:24392163, PubMed:27226539). Required to ensure correct topology and ER insertion of CAMLG (PubMed:31417168, PubMed:32187542). (updated: June 2, 2021)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs35946782 |
No binding partner found
Biological Process
Otic vesicle development
Posttranslational protein targeting to endoplasmic reticulum membrane
Protein insertion into ER membrane
Protein stabilization
Sensory perception of sound
Synapse organization
Tail-anchored membrane protein insertion into ER membrane
Cellular Component
Endoplasmic reticulum
Endoplasmic reticulum membrane
GET complex
Integral component of membrane
Nucleus
Molecular Function
The reference OMIM entry for this protein is 602915
Tryptophan-rich basic protein; wrb
Congenital heart disease 5 gene, formerly; chd5, formerly
CLONING
Egeo et al. (1998) used direct cDNA selection to identify over 300 fetal heart and cultured cardiac fibroblast cDNAs from chromosome 21q22.2-q22.3 in order to screen for genes that may contribute to congenital heart defects in Down syndrome (190685) patients. One clone, designated WRB, encoded a predicted 174-amino acid protein with a pI of 10.5. The WRB protein has a tryptophan-rich C-terminal region and a potential nuclear localization signal. By immunofluorescence, Egeo et al. (1998) localized WRB to the cell nucleus. Northern blot analysis of human adult and fetal tissues showed wide expression of WRB as 1.4- and 1.6-kb mRNAs.MAPPING
By analysis of genomic clones and somatic cell hybrids, Egeo et al. (1998) mapped the WRB gene to chromosome 21q22.3, near HMG14 (163920). ... More on the omim web site
July 1, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
April 25, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 602915 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).