Guanine nucleotide exchange factor DBS (MCF2L)
The protein contains 1137 amino acids for an estimated molecular weight of 128109 Da.
Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42 (PubMed:15157669).', 'Does not catalyze guanine nucleotide exchange on CDC42 (PubMed:15157669). (updated: Dec. 11, 2019)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.
(right-click above to access to more options from the contextual menu)
Biological Process
G protein-coupled receptor signaling pathway
Intracellular signal transduction
Positive regulation of apoptotic process
Positive regulation of Rho protein signal transduction
Regulation of Rho protein signal transduction
Regulation of small GTPase mediated signal transduction
The reference OMIM entry for this protein is 609499
Mcf2-like protein; mcf2l
Ost
Kiaa0362
CLONING
By screening a rat osteosarcoma cDNA expression library for clones that induced transformation in a mouse fibroblast cell line, Horii et al. (1994) cloned rat Mcf2l, which they designated Ost. The deduced 100-kD protein contains a central DBL (MCF2; 311030) homology (DH) domain and a pleckstrin (PLEK; 173570) homology (PH) domain. Northern blot analysis detected Ost predominantly in rat brain and heart. Immunocytochemical localization of Ost in rat brain sections revealed expression in neurons and alpha-tanycytes. The protein was serine phosphorylated and localized in the cytoplasm of fractionated transfected mouse fibroblasts. By sequencing clones obtained from a size-fractionated human brain cDNA library, Nagase et al. (1997) cloned MCF2L, which they designated KIAA0362. The deduced 1,108-amino acid protein shares 87.5% identity with rat Ost. RT-PCR analysis detected high MCF2L expression in placenta, brain, and kidney, intermediate expression in prostate, lung, and liver, and low expression in heart, skeletal muscle, pancreas, thymus, ovary, and small intestine. No expression was detected in spleen and testis.GENE FUNCTION
Horii et al. (1994) found that transfection of a mouse fibroblast cell line with full-length rat Ost resulted in the appearance of morphologically transformed foci. The DH and PH domains of Ost were required for transforming activity, and removal of an N-terminal domain increased Ost transforming ability. Purified rat Ost protein catalyzed guanine nucleotide exchange on RhoA (ARHA; 165390) and Cdc42 (116952), but not on any other Rho and Ras (see 190020) family members tested. Ost did not appear to associate with RhoA or Cdc42, but interacted specifically with the GTP-bound form of Rac1 (602048).MAPPING
By radiation hybrid analysis, Nagase et al. (1997) mapped the MCF2L gene to chromosome 13. ... More on the omim web site
Jan. 22, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 609499 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).