Vesicle-associated membrane protein 5 (VAMP5)

The protein contains 116 amino acids for an estimated molecular weight of 12805 Da.

 

May participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking. (updated: Sept. 12, 2018)

Protein identification was indicated in the following studies:

  1. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 78%
Model score: 0
No model available.

(right-click above to access to more options from the contextual menu)

The reference OMIM entry for this protein is 607029

Vesicle-associated membrane protein 5; vamp5
Myobrevin

CLONING

By database searching with the mouse synaptobrevin/Vamp2 (185881) sequence as query, Zeng et al. (1998) identified a mouse EST clone for Vamp5. The deduced 102-amino acid protein is predicted to contain a cytoplasmic coiled-coil structure at the N terminus, a hydrophobic domain, and a short hydrophilic C-terminal sequence. Vamp5 shares 33% sequence identity with cellubrevin (VAMP3; 603657) and synaptobrevin/Vamp2. Northern blot analysis detected a major 700-bp transcript in skeletal muscle and heart, with much lower expression in spleen, lung, liver, kidney, and testis, and no expression in brain. An approximately 1.5-kb transcript was detected in testis. By Northern blot analysis of mouse muscle cells in culture, Zeng et al. (1998) found that Vamp5 expression was 8- to 10-fold higher in multinucleated myotubes than in undifferentiated myoblasts. Western blot analysis revealed protein levels 6-fold higher in myotubes than in myoblasts. Immunofluorescence and electron microscopy indicated that Vamp5 localizes to intracellular perinuclear and peripheral vesicular structures of myotubes as well as to the plasma membrane. Vamp5 staining did not colocalize with a Golgi marker, GS28 (GOSR1; 604026), upon treatment of the cells with Golgi-disrupting agents.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the VAMP5 gene to chromosome 2 (TMAP SGC32754). ... More on the omim web site

Subscribe to this protein entry history

Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 607029 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).