Nucleobindin-1 (NUCB1)
The protein contains 461 amino acids for an estimated molecular weight of 53879 Da.
Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity). (updated: Dec. 11, 2019)
Protein identification was indicated in the following studies:
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs35456905 | |
| dbSNP:rs200372110 |
Biological Process
Cellular protein metabolic process
Post-translational protein modification
Regulation of protein targeting
Response to cisplatin
Small GTPase mediated signal transduction
Cellular Component
Cis-Golgi network
Early endosome
Endoplasmic reticulum lumen
Endoplasmic reticulum-Golgi intermediate compartment
Extracellular exosome
Extracellular space
Extrinsic component of Golgi membrane
Golgi cisterna membrane
Golgi-associated vesicle
Lumenal side of Golgi membrane
Membrane
Nucleus
Rough endoplasmic reticulum
Trans-Golgi network
The reference OMIM entry for this protein is 601323
Nucleobindin 1; nucb1
Nuc
CLONING
Nucleobindin (Nuc) was first identified as a secreted protein of 55 kD that promotes production of DNA-specific antibodies in lupus-prone mice with the lymphoproliferation (lpr) mutation (Kanai et al., 1993). These mice produce large amounts of antibody against both single-stranded and double-stranded DNA (Theofilopoulos and Dixon, 1985). The primary defect is a deficiency in expression of the Fas gene (134637). Analysis of cDNA that encodes mouse Nuc demonstrated that the protein is composed of a signal peptide, a DNA-binding site, 2 calcium-binding motifs, and a leucine zipper (Miura et al., 1992). Miura et al. (1996) cloned human NUC, which encodes a deduced 461-amino acid peptide. A 2.4-kb NUC transcript was expressed in all organs examined.GENE STRUCTURE
Miura et al. (1996) analyzed the organization of the human NUC gene. It consists of 13 exons that are distributed in a region of 32 kb. The functional motifs identified in the murine protein are encoded in corresponding human exons. Comparison of nucleotide sequences in the promoter regions between human and mouse NUC genes revealed several conserved sequences. The promoter is of the TATA-less type, and transcription starts at multiple sites in both the human and the mouse genes. These features suggested to Miura et al. (1996) that NUC may play a role as a housekeeping gene.MAPPING
By PCR analysis of human/hamster somatic cell hybrids and by fluorescence in situ hybridization, Miura et al. (1996) mapped the NUC gene to 19q13.2-q13.4. ... More on the omim web site
Jan. 22, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: OMIM entry 601323 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).