Filamin A-interacting protein 1-like (FILIP1L)

The protein contains 1135 amino acids for an estimated molecular weight of 130382 Da.

 

Acts as a regulator of the antiangiogenic activity on endothelial cells. When overexpressed in endothelial cells, leads to inhibition of cell proliferation and migration and an increase in apoptosis. Inhibits melanoma growth When expressed in tumor-associated vasculature. (updated: Sept. 12, 2018)

Protein identification was indicated in the following studies:

  1. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 0%
Model score: 34

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VariantDescription
dbSNP:rs793440
dbSNP:rs28362487

No binding partner found

Biological Process

Cellular Component

Cytoplasm GO Logo
Membrane GO Logo
Nucleus GO Logo

Molecular Function

The reference OMIM entry for this protein is 612993

Filamin a-interacting protein 1-like; filip1l

CLONING

Kwon et al. (2008) stated that the 893-amino acid FILIP1L protein shares 46% identity with FILIP1 (607307). FILIP1L contains an extended coiled-coil region in its N-terminal half that includes 2 leucine zipper motifs and a prefoldin (see 604897) domain. The N-terminal half of FILIP1L shares significant similarity with bacterial SbcC, an ATPase involved in DNA repair, and the C-terminal half of FILIP1L shares similarity with herpes virus major outer envelope glycoprotein. A second FILIP1L isoform has an N-terminal extension and contains 1,135 amino acids. Western blot analysis of fractionated human umbilical vein endothelial cells (HUVECs) detected FILIP1L at an apparent molecular mass of 110 kD predominantly in the cytoplasm, with less expression in membrane and nuclear fractions. Immunohistochemical analysis of normal human colon tissue detected FILIP1L in the vasculature and muscularis mucosa. In colon cancer, FILIP1L was expressed in the vasculature and tumor stroma. Kwon et al. (2008) noted that FILIP1L orthologs have only been found in mammals.

GENE FUNCTION

Kwon et al. (2008) found that expression of FILIP1L was upregulated in HUVECs following treatment with angiogenesis inhibitors, with strong punctate FILIP1L staining in the cytoplasm. Overexpression of FILIP1L in endothelial cells led to inhibition of cell proliferation and increased apoptosis. Truncation of FILIP1L after residue 790 increased its ability to inhibit cell proliferation. Expression of truncated FILIP1L in human melanoma cells inhibited tumor growth following injection in nude mice. Tumors of these mice showed reduced vascular density and extensive apoptosis compared with tumors grown from cells lacking truncated FILIP1L.

MAPPING

Kwon et al. (2008) stated that the FILIP1L gene maps to chromosome 3q12.1. ... More on the omim web site

Subscribe to this protein entry history

Feb. 23, 2019: Protein entry updated
Automatic update: model status changed

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).

Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 612993 was added.