Golgin subfamily A member 1 (GOLGA1)
The protein contains 767 amino acids for an estimated molecular weight of 88184 Da.
Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs35237091 | |
| dbSNP:rs583134 | |
| dbSNP:rs634710 |
Biological Process
Cellular Component
Acrosomal vesicle
Cytosol
Golgi apparatus
Golgi membrane
Perinuclear region of cytoplasm
Trans-Golgi network
Molecular Function
The reference OMIM entry for this protein is 602502
Golgi autoantigen, golgin subfamily a, 1; golga1
Golgin 97
CLONING
Griffith et al. (1997) used serum from a Sjogren syndrome (270150) patient with scleroderma to isolate a cDNA strand encoding a Golgi complex antigen from a HeLa cell cDNA library. The clone encoded a predicted 767-amino acid protein with a pI of 5.09, a granin motif (see 113705), and coiled-coil domains. On Western blots of HeLa cell extracts, the encoded protein migrated at 97 kD. Antibodies to the protein, which Griffith et al. (1997) named 'golgin-97,' stained the Golgi complex.GENE FUNCTION
Wong and Munro (2014) selected 10 mammalian golgins that are conserved outside of vertebrates and found on different regions of the Golgi and ectopically expressed them at the mitochondria through attachment to a mitochondrial transmembrane domain in place of their C-terminal Golgi targeting domain. The authors then used the distribution of cargo-laden vesicles originating from different locations as a readout for the golgins' tethering activity. Wong and Munro (2014) found that golgin-97 (GOLGA1), golgin-245 (GOLGA4; 602509), and GCC88 (607418) were able to capture endosome-to-Golgi cargoes; GM130 (GOLGA2; 602580) and GMAP210 (TRIP11; 604505) were able to capture endoplasmic reticulum (ER)-to-Golgi cargoes; and golgin-84 (GOLGA5; 606918), TMF1 (601126), and GMAP210 were able to capture Golgi resident proteins. Furthermore, electron microscopy yielded ultrastructural evidence for the accumulation of vesicular membranes around mitochondria decorated with specific golgins. Wong and Munro (2014) concluded that these data suggested that not only do the golgins capture vesicles, they also exhibit specificity toward vesicles of different origins: from the endosomes, from the ER, or from within the Golgi itself. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 602502 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).