Prenylcysteine oxidase 1 (PCYOX1)
The protein contains 505 amino acids for an estimated molecular weight of 56640 Da.
Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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| Variant | Description |
|---|---|
| dbSNP:rs2706762 | |
| dbSNP:rs17005441 | |
| dbSNP:rs34041544 |
Biological Process
Chloride transport
Prenylated protein catabolic process
Prenylcysteine catabolic process
Prenylcysteine metabolic process
The reference OMIM entry for this protein is 610995
Prenylcysteine oxidase 1; pcyox1
Prenylcysteine lyase; pcl1
Kiaa0908
DESCRIPTION
Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product (Tschantz et al., 1999).CLONING
By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned PCYOX1, which they called KIAA0908. The 3-prime untranslated region contains several SINEs, including Alu and MIR sequences. The predicted protein has 506 amino acids. RT-PCR ELISA detected high to moderate expression in all adult and fetal human tissues and all specific brain regions tested. Using oligonucleotide probes derived from bovine Pcyox1 to screen a human brain cDNA library, followed by 5-prime RACE, Tschantz et al. (1999) cloned PCYOX1. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and potential N-glycosylation sites. Northern blot analysis detected a 6-kb transcript in heart, kidney, liver, colon, placenta, skeletal muscle, and pancreas, with low expression in brain and no expression in spleen. By endoglycosidase H treatment and SDS-PAGE analysis of in vitro translated PCYOX1, Tschantz et al. (1999) showed that PCYOX1 is processed by removal of a signal peptide and N-glycosylation. Immunofluorescence studies of PCYOX1 showed a nonnuclear punctate pattern, and PCYOX1 colocalized with lysosomal marker LAMP1 (153330) in HEK293 cells.MAPPING
By radiation hybrid analysis, Nagase et al. (1998) mapped the PCYOX1 gene to chromosome 2. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 610995 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).