Annexin A4 (ANXA4)

The protein contains 319 amino acids for an estimated molecular weight of 35883 Da.

 

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  4. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology.


Interpro domains
Total structural coverage: 100%
Model score: 100
No model available.

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VariantDescription
dbSNP:rs2228203

The reference OMIM entry for this protein is 106491

Annexin a4; anxa4
Annexin iv; anx4
Placental anticoagulant protein ii
Zymogen granule membrane-associated protein, 36-kd; zap36

CLONING

The annexins, or lipocortins, are a family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV, otherwise known as placental anticoagulant protein II, was 1 of the 4 annexins isolated from human placenta on the basis of their in vitro anticoagulant activity. Grundmann et al. (1988) reported a cDNA clone for annexin IV. Hauptmann et al. (1989) showed that annexin IV has 45 to 59% identity with other members of the annexin family. Fukuoka et al. (2002) found that rat Anxa4, which they called Zap36, was ubiquitously expressed in epithelial tissues. In dog and rat pancreas, Zap36 associated with zymogen granule membranes.

GENE STRUCTURE

By genomic Southern blotting with a cDNA probe, Tait et al. (1992) determined that the ANXA4 gene spans 18 to 56 kb. DNA sequence analysis demonstrated a single intron with exon-intron boundaries in exactly the same position as in the mouse annexin I and annexin II genes.

MAPPING

By PCR analysis of somatic cell hybrids and in situ hybridization with a cDNA probe, Tait et al. (1992) mapped the human ANXA4 gene to chromosome 2p13. Barrow et al. (1994) demonstrated that the Anxa4 gene is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 106491 was added.

Jan. 27, 2016: Protein entry updated
Automatic update: model status changed

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed