60S ribosomal protein L35a (RPL35A)
The protein contains 110 amino acids for an estimated molecular weight of 12538 Da.
Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| DBA5 |
Biological Process
Cellular protein metabolic process
Cytoplasmic translation
Gene expression
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Ribosomal large subunit biogenesis
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational elongation
Translational initiation
Translational termination
Viral life cycle
Viral process
Viral transcription
The reference OMIM entry for this protein is 180468
Ribosomal protein l35a; rpl35a
DESCRIPTION
In eukaryotes, the ribosome is composed of 4 different ribosomal RNA species and 79 ribosomal proteins, including RPL35A (Boria et al., 2010).CLONING
Boria et al. (2010) stated that the RPL35A gene encodes a 110-amino acid protein with a molecular mass of 12.4 kD.GENE STRUCTURE
Boria et al. (2010) stated that the RPL35A gene contains 6 exons spanning 5.6 kb. The first exon is noncoding.MAPPING
By fluorescence in situ hybridization, Colombo et al. (1996) mapped the RPL35A to chromosome 3q29-qter. They confirmed the localization by PCR analysis of a hybrid cell line containing only chromosome 3.GENE FUNCTION
Farrar et al. (2008) tested small hairpin RNAs (shRNA) targeting RPL35A mRNA in UT-7/Epo and TF-1 cell lines and demonstrated that RPL35A is essential for maturation of 28S and 5.8S rRNAs, 60S subunit biogenesis, normal proliferation, and cell survival.MOLECULAR GENETICS
Using a candidate gene strategy combining high-resolution genomic mapping and gene expression microarray, Farrar et al. (2008) analyzed the chromosome 3q29-qter and 3q29 deletions, respectively, from a boy and an unrelated girl with Diamond-Blackfan anemia (DBA5; 612528), and identified RPL35A as a potential DBA gene. Screening genomic DNA from 148 additional probands with DBA, the authors identified 3 heterozygous mutations (180468.0001-180468.0003), yielding an estimated 3.3% rate of RPL35A abnormalities in DBA probands. Analysis of pre-rRNA processing in primary DBA lymphoblastoid cell lines demonstrated alterations of large ribosomal subunit rRNA in both RPL35A-mutated and RPL35A-wildtype DBA patients, suggesting that additional large ribosomal subunit gene defects are likely present in some cases of DBA.HISTORY
Using a PCR product for the analysis of rodent/human somatic cell hybrids, Feo et al. (1992) incorrectly mapped the RPL35A gene to chromosome 18. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 16, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 26, 2017: Protein entry updated
Automatic update: model status changed
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 180468 was added.
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed