ADP-ribosylation factor 4 (ARF4)

The protein contains 180 amino acids for an estimated molecular weight of 20511 Da.

 

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is annotated as membranous in Gene Ontology.


Interpro domains
Total structural coverage: 100%
Model score: 100
No model available.

(right-click above to access to more options from the contextual menu)

VariantDescription
dbSNP:rs11550597

The reference OMIM entry for this protein is 601177

Adp-ribosylation factor 4; arf4
Adp-ribosylation factor 2, formerly; arf2, formerly

CLONING

Monaco et al. (1990) used degenerate PCR with genomic DNA to identify a member of the human ADP-ribosylation factor (ARF) gene family. The gene, designated ARF4, encodes a predicted 180-amino acid protein that is approximately 80% identical to the other human ARFs (see, for example, 103180) and 73% identical to yeast ARF. By DNA sequence analysis, the authors stated that ARF4 is the most divergent member of the human ARFs. A processed pseudogene that is 94% identical at the nucleotide level was also identified. Northern blot analysis detected a 1.8-kb mRNA in IMR-32 neuroblastoma cells.

MAPPING

By fluorescence in situ hybridization, Vorobieva et al. (1995) mapped the ARF4 gene, which they called ARF2, to chromosome 3p21.2-p21.1. Kim (1998) mapped the ARF4 gene to 3p14.1 by PCR analysis of somatic cell hybrid DNA and radiation hybrid analysis. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601177 was added.

Jan. 27, 2016: Protein entry updated
Automatic update: model status changed

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed