Ribose-phosphate pyrophosphokinase 3 (PRPS1L1)
The protein contains 318 amino acids for an estimated molecular weight of 34839 Da.
Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. (updated: Jan. 7, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs3800962 |
No binding partner found
The reference OMIM entry for this protein is 611566
Phosphoribosylpyrophosphate synthetase 1-like 1; prps1l1
Prps1-like 1
Phosphoribosylpyrophosphate synthetase 3; prps3
CLONING
Using rat Prps1 (311850) to screen a human testis cDNA library, Taira et al. (1990) cloned PRPS1L1, which they called PRPS3. The deduced 318-amino acid protein has a calculated molecular mass of 34.7 kD. Peptide sequencing and mutagenesis experiments showed that translation of PRPS3 initiates at an ACG codon that specifies methionine rather than threonine, and that this methionine is subsequently removed, resulting in a mature protein with an N-terminal proline. In vitro transcription and cell-free translation resulted in a protein with an apparent molecular mass of 38 kD. Northern blot analysis detected a 1.4-kb transcript in human testis. Prps3 expression was not detected in testis of 3-week-old rats, but its expression increased after 4 weeks, roughly correlating with the appearance of primary spermatocytes.MAPPING
By Southern blot and somatic cell hybrid analyses, Taira et al. (1990) mapped the PRPS1L1 gene to chromosome 7. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for PRPS1L1
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611566 was added.