Cysteine and glycine-rich protein 1 (CSRP1)
The protein contains 193 amino acids for an estimated molecular weight of 20567 Da.
Could play a role in neuronal development. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs3738283 |
The reference OMIM entry for this protein is 123876
Cysteine- and glycine-rich protein 1; csrp1
Cysteine-rich protein 1; crp1
Cysteine-rich protein; csrp; crp
DESCRIPTION
The human gene encoding cysteine-rich protein (CSRP) is a highly conserved, cell cycle-regulated gene that is induced in the immediate early response to serum repletion in serum-starved, noncycling cells. The LIM/double zinc finger motif found in cysteine-rich protein is found in an expanding group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation (summary by Wang et al., 1992).CLONING
By screening a human placenta cDNA library with a human prolactin (PRL; 176760) cDNA, Liebhaber et al. (1990) isolated cDNAs encoding CRP. The deduced 193-amino acid protein has 2 repeats of a domain that consists of 2 putative zinc fingers immediately followed by a glycine-rich motif containing a high proportion of aromatic and basic residues. CRP does not share regions of significant structural similarity with PRL. Northern blot and RT-PCR analyses detected CRP expression in all human nucleated tissues and cell lines examined; CRP is expressed as a 1.8-kb transcript. Wang et al. (1992) cloned the human CRP genomic sequence. They showed that CRP is a primary response gene in both human fibroblasts and mouse Balb/c 3T3 cells; in the mouse cells, the kinetic profile of its induction closely paralleled that of c-myc (190080).GENE STRUCTURE
Wang et al. (1992) determined that the CRP gene contains 6 exons, with a 10.4-kb first intron, and spans approximately 23.2 kb from the cap site to the polyadenylation site.GENE FAMILY
Other members of the LIM/double zinc finger group include cysteine-rich intestinal protein (CRIP; 123875), CSRP2 (601871), CSRP3 (600824), and the rhombotin genes RBTN1 (186921), RBTN2 (180385), and RBTN3 (180386). Weiskirchen et al. (1995) described the CRP family of LIM domain proteins.MAPPING
Using a panel of human/rodent somatic cell hybrids, Wang et al. (1992) assigned the CSRP gene to chromosome 1. By in situ hybridization of (3)H-labeled cDNA, they regionalized the gene to 1q24-q32. A common MspI polymorphism was found and mapped to intron 4 of the CSRP gene. By FISH, Erdel and Weiskirchen (1998) mapped the CSRP1 to 1q32. Alli and Consalez (1998) mapped the Csrp gene to mouse chromosome 3 by haplotype and linkage analysis of progeny from an interspecific backcross panel. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 123876 was added.