Elongation factor 1-beta (EEF1B2)
The protein contains 225 amino acids for an estimated molecular weight of 24764 Da.
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cellular protein metabolic process
Gene expression
Response to ethanol
Translation
Translational elongation
Molecular Function
Guanyl-nucleotide exchange factor activity
Translation elongation factor activity
The reference OMIM entry for this protein is 600655
Eukaryotic translation elongation factor 1, beta-2; eef1b2
Elongation factor 1, beta-2a
Elongation factor 1, beta; ef1b eukaryotic translation elongation factor 1, beta-1, pseudogene, included; eef1b1, included; eef1b2p1, included
Eukaryotic tr
DESCRIPTION
Eukaryotic elongation factor-1 (EF1) consists of 4 subunits, EF1-alpha (EEF1A1; 130590), EF1-beta, EF1-gamma (EEF1G; 130593), and EF1-delta (EEF1D; 130592). EIF-alpha-GTP transfers aminoacyl-tRNA to the ribosome, and the release of animoacyl-tRNA from EIF-alpha-GTP is driven by GTP hydrolysis. EF1-alpha-GDP is recycled to EF1-alpha-GTP by the EF1-beta, -gamma, and -delta subunits (Sanders et al., 1996).CLONING
Sanders et al. (1991) identified a human EF1-beta cDNA by hybridization with a pig EF1-beta probe. By immunofluorescence analysis, Sanders et al. (1996) found that EF1-beta, -gamma, and -delta showed a perinuclear distribution and colocalized with an endoplasmic reticulum resident protein in human foreskin fibroblasts. In contrast, EF1-alpha showed strong nuclear staining and diffuse cytoplasmic staining.MAPPING
The EEF1B2 gene was mapped by Pizzuti et al. (1993) to chromosome 2 by PCR analysis of a somatic cell hybrid DNA panel. - Pseudogenes From a cDNA library of human ovarian granulosa cells, von der Kammer et al. (1991) isolated a cDNA that appeared to code for human EF1-beta. The gene corresponding to this cDNA was mapped to chromosome 15 by PCR analysis of a chromosomal hybrid panel by Pizzuti et al. (1993). Chambers et al. (2001) determined that the gene isolated by von der Kammer et al. (1991) is actually a processed pseudogene on chromosome 15 corresponding to an alternative splice form of EEF1B2. Two additional pseudogenes, previously designated EEF1B3 AND EEF1B4, were mapped to chromosome 5 and chromosome X, respectively, by Pizzuti et al. (1993). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 600655 was added.
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed