60S ribosomal protein L12 (RPL12)
The protein contains 165 amino acids for an estimated molecular weight of 17819 Da.
Binds directly to 26S ribosomal RNA. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cellular protein metabolic process
Cytoplasmic translation
Gene expression
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Ribosomal large subunit assembly
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational elongation
Translational initiation
Translational termination
Viral life cycle
Viral process
Viral transcription
The reference OMIM entry for this protein is 180475
Ribosomal protein l12; rpl12
CLONING
Biogenesis of the mammalian ribosome, the site of protein synthesis, involves coordinated expression of 4 ribosomal RNA molecules and approximately 75 ribosomal proteins. Chu et al. (1993) isolated a cDNA clone encoding human ribosomal protein L12 from a dermal microvascular endothelial cell cDNA library. The protein contains 165 amino acids and exhibits 99.4% sequence identity with rat ribosomal protein L12. Northern blot analysis detected an mRNA transcript of 800 bases. Cuccurese et al. (2005) identified an RPL12 splice variant in a human lung carcinoma cell line that was induced following inhibition of nonsense-mediated mRNA decay. The variant retains part of intron 1, resulting in a premature termination codon. Northern blot analysis showed that the variant is 0.8 kb, while the major species is 0.63 kb. Intron 1 is highly conserved in human, mouse, and bovine RPL12 genes.MAPPING
Hartz (2007) mapped the RPL12 gene to chromosome 9q33.3 based on an alignment of the RPL12 sequence (GenBank GENBANK AB209471) with the genomic sequence (build 36.1). ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 16, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 26, 2017: Protein entry updated
Automatic update: model status changed
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 180475 was added.
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed