Eukaryotic translation initiation factor 3 subunit F (EIF3F)

The protein contains 357 amino acids for an estimated molecular weight of 37564 Da.

 

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).', 'Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. (updated: Dec. 20, 2017)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 92%
Model score: 0

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VariantDescription
dbSNP:rs1043738
dbSNP:rs1044058
MRT67

The reference OMIM entry for this protein is 603914

Eukaryotic translation initiation factor 3, subunit f; eif3f
Eif3-p47
Eukaryotic translation initiation factor 3, subunit 5, formerly; eif3s5, formerly

DESCRIPTION

Eukaryotic initiation factor-3 (eIF3) is the largest of the eIFs and consists of at least 10 nonidentical subunits, including p47 (EIF3F), in mammals (Asano et al., 1997). For further background information on eIF3, see p66 (EIF3S7; 603915).

CLONING

By searching EST databases with the partial protein sequences of rabbit p66, p47, and p40 (603912), Asano et al. (1997) identified cDNAs encoding the human homologs. Although the predicted 357-amino acid human p47 protein has a calculated molecular mass of 37.5 kD, it migrates at 47 kD by SDS-PAGE. The authors attributed the anomalous migration to the high proline content of the N-terminal region of p47. Sequence analysis revealed that the N-terminal halves of p40 and p47 are similar to each other and are related to the mouse Mov34 protein (157970). Northern blot analysis revealed that p47 is expressed as a 1.3-kb mRNA in HeLa cells.

MAPPING

Hartz (2012) mapped the EIF3F gene to chromosome 11p15.4 based on an alignment of the EIF3F sequence (GenBank GENBANK BC000490) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 17, 2018: Protein entry updated
Automatic update: model status changed

Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 27, 2017: Protein entry updated
Automatic update: model status changed

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603914 was added.

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed