Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3)
The protein contains 346 amino acids for an estimated molecular weight of 36926 Da.
Involved in the splicing process and participates in early heat shock-induced splicing arrest. Due to their great structural variations the different isoforms may possess different functions in the splicing reaction. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| dbSNP:rs2273903 | |
| dbSNP:rs16925347 |
The reference OMIM entry for this protein is 602324
Heterogeneous nuclear ribonucleoprotein h3; hnrnph3
Hnrph3
2h9
CLONING
The RNAs found in the nucleus include pre-mRNAs, mature mRNAs, and intermediate reaction products. Collectively these RNAs are termed heterogeneous nuclear RNAs (hnRNAs). Ribonucleoprotein (RNP) complexes consisting of hnRNAs and associated proteins are responsible for RNA splicing and processing. Mahe et al. (1997) used antibodies against ribonucleoprotein complexes to clone the gene of 1 such protein, which the authors termed 2H9, from a human HeLa cell cDNA library. The sequence of 2H9 predicts a 346-amino acid polypeptide containing 2 putative RNA binding domains. The 2H9 gene shares significant homology with other RNPs, including HNRPH1 (601035), HNRPH2 (300610), and HNRPF (601037). Specific antibodies against 2H9 can inhibit in vitro splicing, suggesting that 2H9 is involved in this process. Immunofluorescence staining revealed that 2H9 is localized to the nucleus, particularly in nuclear bodies.MAPPING
Mahe et al. (1997) used isotopic in situ hybridization to map the 2H9 gene to human chromosome 10q22. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 602324 was added.