Beta-centractin (ACTR1B)
The protein contains 376 amino acids for an estimated molecular weight of 42293 Da.
Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11547231 | |
| dbSNP:rs11692435 |
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II
Establishment of mitotic spindle orientation
Neutrophil degranulation
Nuclear migration along microtubule
The reference OMIM entry for this protein is 605144
Actin-related protein 1b; actr1b
Arp1b
Centractin, beta
Ctrn2
CLONING
Isoforms of actin (e.g., ACTG1; 102560), in association with myosin motor proteins (e.g., MYO1A; 601478), are required for cellular motile processes. In addition to conventional actins, there are several actin-related proteins (e.g., ACTR2; 604221). By searching an EST database and by screening a testis cDNA library, Clark et al. (1994) isolated cDNAs encoding ACTR1B, which they called beta-centractin. The deduced 376-amino acid ACTR1B protein and the ACTR1A protein (605143) are of equal length, and they share 90% amino acid identity and 96% amino acid similarity. Northern blot analysis detected a 2.0-kb ACTR1B transcript at variable levels in all tissues tested. Two-dimensional immunoblot analysis determined that ACTR1B is expressed in the cytosol as part of the dynactin complex, an activator of dynein-driven vesicle movement (see 601143), as a 43-kD protein with a pI of 6.4; levels of ACTR1B were at least 15-fold lower than those of ACTR1A.MAPPING
By somatic cell hybrid analysis, Elsea et al. (1999) mapped the ACTR1B gene to chromosome 2. They localized the ACTR1B gene to 2q11.1-q11.2 using FISH. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 605144 was added.