40S ribosomal protein S10 (RPS10)

The protein contains 165 amino acids for an estimated molecular weight of 18898 Da.

 

Component of the 40S ribosomal subunit. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  6. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 100%
Model score: 100

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The reference OMIM entry for this protein is 603632

Ribosomal protein s10; rps10

DESCRIPTION

The mammalian ribosome is a macromolecular assembly of 4 RNA species (see 180450) and approximately 80 different proteins (see 180466).

CLONING

Adams et al. (1992) isolated an RPS10 cDNA as a human brain EST. By searching sequence databases with the partial sequences of randomly selected cDNAs from a human colorectal cDNA library, Frigerio et al. (1995) identified cDNAs encoding homologs of rat ribosomal proteins S5 (RPS5; 603630), S9 (RPS9; 603631), S10 (RPS10), S29 (RPS29; 603633), L5 (RPL5; 603634), L21 (RPL21; 603636), L27a (RPL27A; 603637), and L28 (RPL28; 603638). Frigerio et al. (1995) completed the cDNA sequences of these human ribosomal proteins. The deduced 165-amino acid human RPS10 differs from rat Rps10 by 2 amino acids; neither protein contains cysteine residues. Northern blot analysis suggested variable expression of RPS10 in colorectal cancers compared to adjacent normal tissues, although no correlation between the level of expression and the severity of the disease was found.

GENE STRUCTURE

Boria et al. (2010) stated that the RPS10 gene contains 6 exons spanning 8.65 kb.

MAPPING

By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi et al. (1998) mapped the human RPS10 gene to chromosome 6p. Boria et al. (2010) stated that the RPS10 gene maps to chromosome 6p21.31.

GENE FUNCTION

Using siRNA knockdown in HeLa cells to analyze the role of RPS10 in pre-rRNA processing, Doherty et al. (2010) found that depletion of RPS10 led to decreased levels of 18S rRNA, indicating that RPS10 is necessary for production of the small subunit. RNA blot analysis showed accumulation of 43S, 26S, and 18S-E pre-rRNAs, consistent with defects in cleavage at both ends of the 18S rRNA.

MOLECULAR GENETICS

Doherty et al. (2010) sequenced 35 ribosomal protein genes in a cohort of 117 patients with Diamond-Blackfan anemia (see DBA9, 613308) who were negative for mutation in 7 known DBA genes and identified 3 mutations in the RPS10 gene (603632.0001-603632.0003) in 5 patients. ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 16, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 26, 2017: Protein entry updated
Automatic update: model status changed

March 25, 2017: Additional information
No protein expression data in P. Mayeux work for RPS10

March 15, 2016: Protein entry updated
Automatic update: OMIM entry 603632 was added.

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed