F-actin-capping protein subunit beta (CAPZB)
The protein contains 277 amino acids for an estimated molecular weight of 31350 Da.
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Actin cytoskeleton organization
Antigen processing and presentation of exogenous peptide antigen via MHC class II
Barbed-end actin filament capping
Blood coagulation
Cell morphogenesis
Cytoskeleton organization
Endoplasmic reticulum to Golgi vesicle-mediated transport
Movement of cell or subcellular component
Negative regulation of filopodium assembly
Regulation of cell morphogenesis
Regulation of lamellipodium assembly
The reference OMIM entry for this protein is 601572
Capping protein, beta; capzb
Cap protein, actin, beta; cappb
CLONING
CapZ was identified in chicken as an actin-binding protein composed of alpha (see 601571) and beta subunits. Barron-Casella et al. (1995) isolated human retinal cDNAs homologous to the beta subunit of chicken CapZ. The derived human beta subunit is 99% identical to chicken subunit residues 1-243. The remaining portion of the human beta subunit (amino acids 244-272) diverges significantly with only 8 out of 29 C-terminal amino acids conserved between the 2 species.MAPPING
Barron-Casella et al. (1995) mapped the CAPZB gene to chromosome 1p36.1 by fluorescence in situ hybridization.HISTORY
In the course of mapping 39 ESTs on chromosome 6p24-p23, Olavesen et al. (1997) concluded that CAPZB is located in that region flanked by EDN1 (131240) on the centromeric side and TFAP2 (107580) on the telomeric side. However, Cooper (1999) stated that a 'simple clerical error' occurred at the outset of this project; the EST in question does not share sequence similarity with the CAPZB gene and, therefore, the mapping of the CAPZB gene to chromosome 6p24-p23 is incorrect. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601572 was added.