Nuclear autoantigenic sperm protein (NASP)

The protein contains 788 amino acids for an estimated molecular weight of 85238 Da.

 

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. (updated: Jan. 31, 2018)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 12%
Model score: 37

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VariantDescription
dbSNP:rs34618000

The reference OMIM entry for this protein is 603185

Nuclear autoantigenic sperm protein; nasp
N1/n2, xenopus, homolog of

DESCRIPTION

During mammalian spermatogenesis, testis-specific histones (see H1FT; 142712) and other testis- and sperm-specific proteins are expressed. NASP is a testis- and sperm-specific histone-binding protein (O'Rand et al., 1992).

CLONING

O'Rand et al. (1992) cloned NASP cDNA from a human testis cDNA library. The gene encodes a 787-amino acid polypeptide with a predicted mass of 85 kD. The protein sequence is 53% identical to that of the Xenopus ortholog, and 85% identical to that of the rabbit ortholog when the N terminus of the rabbit sequence is aligned with amino acid 101 of the human sequence. Two histone-binding domains are strongly conserved. Northern blot analysis revealed a 3.2-kb mRNA. Immunolocalization experiments demonstrated that in the testis, NASP is found primarily in the cytoplasm of primary spermatocytes and around or within the nuclei of spermatids. NASP is present near the acrosomal region in mature human sperm.

MAPPING

Gross (2014) mapped the NASP gene to chromosome 1p34.1 based on an alignment of the NASP sequence (GenBank GENBANK BC010105) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

Dec. 10, 2018: Protein entry updated
Automatic update: model status changed

Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603185 was added.