Myosin light polypeptide 6 (MYL6)
The protein contains 151 amino acids for an estimated molecular weight of 16930 Da.
Regulatory light chain of myosin. Does not bind calcium. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11553509 | |
| dbSNP:rs1050470 |
Biological Process
Axon guidance
Ephrin receptor signaling pathway
Metabolic process
Muscle contraction
Muscle filament sliding
Skeletal muscle tissue development
The reference OMIM entry for this protein is 609931
Myosin light chain 6, alkali, smooth muscle and nonmuscle; myl6
DESCRIPTION
Myosin is composed of heavy chain (MHC; see 160730) and light chain (MLC) subunits. Alkaline MLCs, such as MYL6, dissociate from MHCs at high pH. MYL6 encodes both smooth muscle and nonmuscle MLC isoforms (Hailstones and Gunning, 1990).CLONING
By screening a genomic library with a chicken Mlc and a partial clone of MYL1 (160780), followed by screening lymphoblast and smooth muscle cDNA libraries, Lenz et al. (1989) cloned 2 MYL6 variants generated by alternative splicing of exon 6. They designated the nonmuscle variant LYM4 and the smooth muscle variant GT6. Both deduced proteins contain 151 amino acids and differ at only 5 C-terminal amino acids. The GT6 transcript also has a longer 3-prime UTR. Northern blot analysis detected a 0.7-kb LYM4 transcript in both HeLa cells and human uterus smooth muscle, whereas a GT6 transcript of similar size was detected in uterus smooth muscle only. By screening a transformed human fibroblast cDNA library with MLC1SA (609930), followed by RT-PCR of human colon RNA, Hailstones and Gunning (1990) cloned the nonmuscle and smooth muscle variants of MYL6, which they designated MLC3NM and MLC3SM, respectively. Both deduced proteins contain 151 amino acids and differ from other MLCs in that they lack highly basic amino aids in their N termini. MLC3NM has a calculated molecular mass of 16.9 kD, whereas MLC3SM has a calculated molecular mass of 17 kD. Expression of MYL6 was highest in fibroblasts and myoblasts, lower in fetal muscle, and lowest in adult muscle. Using glutathione S-transferase-3 (GSTP1; 134660) as probe of a retina cDNA library, Bora et al. (1994) cloned an MYL6 splice variant that they designated ESMLC. The deduced 157-amino acid ESMLC protein is identical to GT6 (Lenz et al., 1989) except that it contains additional N-terminal amino acids. The nucleotide sequence of GSTP1 and ESMLC are 54% identical, but the deduced proteins are only 11% identical. PCR analysis detected ESMLC expression in eye, aorta, and heart.GENE STRUCTURE
Lenz et al. (1989) determined that the MYL6 gene contains 7 exons. Exon 1 encodes only the first methionine and the 5-prime UTR.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the MYL6 gene to chromosome 12 (TMAP WI-18807). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for MYL6
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 609931 was added.