ADP-ribosylation factor 3 (ARF3)
The protein contains 181 amino acids for an estimated molecular weight of 20601 Da.
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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Biological Process
Golgi to plasma membrane transport
Intracellular protein transport
Phosphatidylinositol biosynthetic process
Phospholipid metabolic process
Protein transport
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
Small GTPase mediated signal transduction
Small molecule metabolic process
Vesicle-mediated transport
The reference OMIM entry for this protein is 103190
Adp-ribosylation factor 3; arf3
For general information on ADP-ribosylation factors, see 103180.
CLONING
Bobak et al. (1989) identified an ARF3 cDNA from a human cerebellum library. Tsai et al. (1991) isolated and characterized the human ARF3 gene. They also showed that two ARF3 mRNAs of 3.7 and 1.2 kb are generated through the use of alternative polyadenylation signals.GENE STRUCTURE
Tsai et al. (1991) found that the ARF3 gene spans approximately 18.3 kb and contains 5 exons. They found multiple transcription start sites and determined that the conserved amino acid sequences involved in guanine nucleotide binding by ARF3 are distributed among separate exons.MAPPING
By PCR screening of a somatic cell hybrid panel and fluorescence in situ hybridization, Hirai et al. (1996) mapped the ARF3 gene to 12q13. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for ARF3
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 103190 was added.