Alexandre G. de Brevern's publications
Year 2013
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Bhaskara R.M., de Brevern A.G., Srinivasan N.#
Understanding the role of domain–domain linkers in the spatial orientation of domains in multi-domain proteins
J Biol Struct Dyn (2013) 31(12):1467-80.
Inter-domain linkers (IDLs)' bridge flanking domains and support inter-domain communication in multi-domain proteins. Their sequence and conformational preferences enable them to carry out varied functions. They also provide sufficient flexibility to facilitate domain motions and, in conjunction with the interacting interfaces, they also regulate the inter-domain geometry (IDG). In spite of the basic intuitive understanding of the inter-domain orientations with respect to linker conformations and interfaces, we still do not entirely understand the precise relationship among the three. We show that IDG is evolutionarily well conserved and is constrained by the domain-domain interface interactions. The IDLs modulate the interactions by varying their lengths, conformations and local structure, thereby affecting the overall IDG. Results of our analysis provide guidelines in modelling of multi-domain proteins from the tertiary structures of constituent domain components.
preprint
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collaboration with Pr. N. Srinivasan of Molecular Biophysics Unit (IISc, India). Funding:- Indo-French collaborative grant (CEFIPRA/IFCPAR 3903-E) [link to the project]
- The Ministry of Research (France)
- The University Paris Diderot, Sorbonne Paris Cite (France)
- The National Institute of Blood Transfusion (INTS, France)
- The National Institute of Health and Medical Research (INSERM, France)
- The Laboratories of Excellence, GR-Ex (France)
- Department of Biotechnology (India)
Alexandre G. de Brevern
Last Modification : January 2014
