Sequence     &     Structural     Information 
	

		
			

				PTM
			

		
		
			

				PTM
				Methylation
			

		

		
			

				Sequence Information
			


		
		
			

				Uniprot AC
				P62805
			

			

				Organism
				HUMAN (Human)
			

			

				Modified Amino Acid
				K(Lys) 21
			

			

				Annotation dbPTM details
				N6,N6,N6-trimethyllysine; alternate.
N6,N6-dimethyllysine; alternate.
N6-methyllysine; alternate.
N6,N6,N6-trimethyllysine
N6,N6-dimethyllysine
N6-acetyllysine
N6-methyllysine
N6-methylated lysine		
			

		

		
			

				Structure Information
			


		
		
			

				PDB ID Code
				3F9X
			

			

				Model
				-
			

			

				Chain
				E
			

			

				Position
				20
			

			

				SCOP Class
				None
			

			

				MODRES
				N-DIMETHYL-LYSINE                        
			

		

		
			

				Related PTM
			


		
		
			

				Found in same PDB Chain
				-
			

			

				Found in same Uniprot AC
Sort by:
PDB ID Code
  (idpdb-chain_aa)
Sequence position
  (aa_idpdb-chain)
Gallery (?)
Slide 1
Slide 2
Example Frame 



Note: As the images production is automatic, the focus on the PTM site could not be the most optimized.
      In these cases it is recommended to use the PyMOL script to visualize the PTM site in 3D.
Alignment
(?) 

	           Numerotation

	(?)       Uniprot Sequence
	(?)                Clustal
	(?)           PDB Sequence
	(?)        PDB Information
	(?)          DSSP Sequence
	(?) Protein Block Sequence
	
         1         2         3         4         5         6         7         8         9         10        
123456789012345678901234567890123456789012345678901234567890123456789012345678901234567890123456789012


MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
               ***** ****                                                                              
---------------AKRHRXVLRD------------------------------------------------------------------------------
               M____H____                                                                              
                CCCCEEECC                                                                              
                ZZdddddZZ

Scripts     (PyMol,     Modeller,     ...)

PyMol 
	Download
Modeller 
	
If you want to use this structure as a template for modelisation with Modeller, you can use the following sequence to build the alignment file "*.ali".

To avoid the classical Modeller error :

			'Number of residues in the alignment and pdb files are different'(see FAQ n°17),

the missing residues, non-classical amino-acids, and sequence gaps have been replaced by a "X" symbol.
XKRHRXVLRD 
1) align this sequence with your sequence to modelised;
2) replace the "X" by "-" in the obtained alignement, then build your "*.ali" file;
3) use Modeller to make your models;
4) And finally use the following information found in the PDB to add this PTM in your models (see FAQ n°8 & 9 and this archive of Modeller usage).
			

			

			
				

MODRES 3F9X MLY E   20  LYS  N-DIMETHYL-LYSINE                                  
SHEET    4   G 4 MLY E  20  LEU E  22  1  O  MLY E  20   N  TYR B 274           
LINK         C   ARG E  19                 N   MLY E  20     1555   1555  1.33  
LINK         C   MLY E  20                 N   VAL E  21     1555   1555  1.32  
SITE     4 AC2 15 HOH B 398  HIS E  18  MLY E  20                               
HETATM 5163  N   MLY E  20      -2.116  -9.862  14.026  1.00 16.35           N  
ANISOU 5163  N   MLY E  20     1792   2139   2283     33    111    -67       N  
HETATM 5164  CA  MLY E  20      -2.368  -9.061  15.213  1.00 14.97           C  
ANISOU 5164  CA  MLY E  20     1664   1848   2175    -19    -23    -20       C  
HETATM 5165  CB  MLY E  20      -3.258  -7.858  14.941  1.00 15.25           C  
ANISOU 5165  CB  MLY E  20     1627   1960   2208    -55    -27    -25       C  
HETATM 5166  CG  MLY E  20      -2.624  -6.940  13.897  1.00 15.67           C  
ANISOU 5166  CG  MLY E  20     1735   1862   2358     46     44    130       C  
HETATM 5167  CD  MLY E  20      -3.639  -5.901  13.450  1.00 15.11           C  
ANISOU 5167  CD  MLY E  20     1673   1659   2410   -170     82    199       C  
HETATM 5168  CE  MLY E  20      -3.076  -5.109  12.295  1.00 15.89           C  
ANISOU 5168  CE  MLY E  20     1768   1823   2445    -61   -121    380       C  
HETATM 5169  NZ  MLY E  20      -2.587  -5.947  11.194  1.00 17.92           N  
ANISOU 5169  NZ  MLY E  20     2063   2474   2272   -516    270    398       N  
HETATM 5170  CH1 MLY E  20      -3.615  -6.924  10.755  1.00 20.11           C  
ANISOU 5170  CH1 MLY E  20     2173   2791   2677   -573     25    241       C  
HETATM 5171  CH2 MLY E  20      -2.232  -5.035  10.081  1.00 19.02           C  
ANISOU 5171  CH2 MLY E  20     2271   2677   2280   -491    385    416       C  
HETATM 5172  C   MLY E  20      -3.007  -9.952  16.238  1.00 14.09           C  
ANISOU 5172  C   MLY E  20     1534   1721   2100     79    -11     73       C  
HETATM 5173  O   MLY E  20      -3.840 -10.813  15.900  1.00 14.38           O  
ANISOU 5173  O   MLY E  20     1450   1614   2398    -48     50    -64       O