Sequence     &     Structural     Information 
	

		
			

				PTM
			

		
		
			

				PTM
				Methylation
			

		

		
			

				Sequence Information
			


		
		
			

				Uniprot AC
				P62805
			

			

				Organism
				HUMAN (Human)
			

			

				Modified Amino Acid
				K(Lys) 21
			

			

				Annotation dbPTM details
				N6,N6,N6-trimethyllysine; alternate.
N6,N6-dimethyllysine; alternate.
N6-methyllysine; alternate.
N6,N6,N6-trimethyllysine
N6,N6-dimethyllysine
N6-acetyllysine
N6-methyllysine
N6-methylated lysine		
			

		

		
			

				Structure Information
			


		
		
			

				PDB ID Code
				3F9X
			

			

				Model
				-
			

			

				Chain
				F
			

			

				Position
				20
			

			

				SCOP Class
				None
			

			

				MODRES
				N-DIMETHYL-LYSINE                        
			

		

		
			

				Related PTM
			


		
		
			

				Found in same PDB Chain
				-
			

			

				Found in same Uniprot AC
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PDB ID Code
  (idpdb-chain_aa)
Sequence position
  (aa_idpdb-chain)
Gallery (?)
Slide 1
Slide 2
Example Frame 



Note: As the images production is automatic, the focus on the PTM site could not be the most optimized.
      In these cases it is recommended to use the PyMOL script to visualize the PTM site in 3D.
Alignment
(?) 

	           Numerotation

	(?)       Uniprot Sequence
	(?)                Clustal
	(?)           PDB Sequence
	(?)        PDB Information
	(?)          DSSP Sequence
	(?) Protein Block Sequence
	
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MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
               ***** ****                                                                              
---------------AKRHRXVLRD------------------------------------------------------------------------------
               M____H___M                                                                              
                CCCCEECC                                                                               
                ZZcdddZZ

Scripts     (PyMol,     Modeller,     ...)

PyMol 
	Download
Modeller 
	
If you want to use this structure as a template for modelisation with Modeller, you can use the following sequence to build the alignment file "*.ali".

To avoid the classical Modeller error :

			'Number of residues in the alignment and pdb files are different'(see FAQ n°17),

the missing residues, non-classical amino-acids, and sequence gaps have been replaced by a "X" symbol.
XKRHRXVLRX 
1) align this sequence with your sequence to modelised;
2) replace the "X" by "-" in the obtained alignement, then build your "*.ali" file;
3) use Modeller to make your models;
4) And finally use the following information found in the PDB to add this PTM in your models (see FAQ n°8 & 9 and this archive of Modeller usage).
			

			

			
				

MODRES 3F9X MLY F   20  LYS  N-DIMETHYL-LYSINE                                  
SHEET    4   C 4 MLY F  20  VAL F  21  1  O  MLY F  20   N  TYR A 274           
LINK         C   ARG F  19                 N   MLY F  20     1555   1555  1.32  
LINK         C   MLY F  20                 N   VAL F  21     1555   1555  1.32  
SITE     5 AC1 18 HIS F  18  MLY F  20                                          
HETATM 5247  N   MLY F  20       0.545   3.890  -7.806  1.00 18.79           N  
ANISOU 5247  N   MLY F  20     2477   2440   2222   -195    174    -43       N  
HETATM 5248  CA  MLY F  20       1.144   2.569  -7.952  1.00 18.39           C  
ANISOU 5248  CA  MLY F  20     2402   2376   2211   -247     91    -68       C  
HETATM 5249  CB  MLY F  20       2.037   2.480  -9.178  1.00 19.30           C  
ANISOU 5249  CB  MLY F  20     2422   2594   2316   -237     92    -34       C  
HETATM 5250  CG  MLY F  20       1.238   2.817 -10.424  1.00 20.53           C  
ANISOU 5250  CG  MLY F  20     2510   2777   2515   -278    208     51       C  
HETATM 5251  CD  MLY F  20       2.168   2.997 -11.630  1.00 22.66           C  
ANISOU 5251  CD  MLY F  20     2795   3040   2773   -315    318     64       C  
HETATM 5252  CE  MLY F  20       1.398   3.583 -12.809  1.00 24.52           C  
ANISOU 5252  CE  MLY F  20     3418   3231   2668    -90    431    -39       C  
HETATM 5253  NZ  MLY F  20       2.215   3.729 -14.042  1.00 26.32           N  
ANISOU 5253  NZ  MLY F  20     3770   3225   3004    109    759    -96       N  
HETATM 5254  CH1 MLY F  20       2.469   2.462 -14.736  1.00 24.47           C  
ANISOU 5254  CH1 MLY F  20     3705   2979   2612    -13    696   -113       C  
HETATM 5255  CH2 MLY F  20       1.450   4.575 -14.950  1.00 28.13           C  
ANISOU 5255  CH2 MLY F  20     4199   3601   2890     13    653    -60       C  
HETATM 5256  C   MLY F  20       1.894   2.331  -6.666  1.00 18.75           C  
ANISOU 5256  C   MLY F  20     2557   2415   2153   -196     60   -138       C  
HETATM 5257  O   MLY F  20       2.501   3.263  -6.132  1.00 18.61           O  
ANISOU 5257  O   MLY F  20     2383   2487   2202   -495     24   -328       O