Sequence     &     Structural     Information 
	

		
			

				PTM
			

		
		
			

				PTM
				Methylation
			

		

		
			

				Sequence Information
			


		
		
			

				Uniprot AC
				P62805
			

			

				Organism
				HUMAN (Human)
			

			

				Modified Amino Acid
				K(Lys) 21
			

			

				Annotation dbPTM details
				N6,N6,N6-trimethyllysine; alternate.
N6,N6-dimethyllysine; alternate.
N6-methyllysine; alternate.
N6,N6,N6-trimethyllysine
N6,N6-dimethyllysine
N6-acetyllysine
N6-methyllysine
N6-methylated lysine		
			

		

		
			

				Structure Information
			


		
		
			

				PDB ID Code
				3F9X
			

			

				Model
				-
			

			

				Chain
				G
			

			

				Position
				20
			

			

				SCOP Class
				None
			

			

				MODRES
				N-DIMETHYL-LYSINE                        
			

		

		
			

				Related PTM
			


		
		
			

				Found in same PDB Chain
				-
			

			

				Found in same Uniprot AC
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PDB ID Code
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Sequence position
  (aa_idpdb-chain)
Gallery (?)
Slide 1
Slide 2
Example Frame 



Note: As the images production is automatic, the focus on the PTM site could not be the most optimized.
      In these cases it is recommended to use the PyMOL script to visualize the PTM site in 3D.
Alignment
(?) 

	           Numerotation

	(?)       Uniprot Sequence
	(?)                Clustal
	(?)           PDB Sequence
	(?)        PDB Information
	(?)          DSSP Sequence
	(?) Protein Block Sequence
	
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MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
               ***** ****                                                                              
---------------AKRHRXVLRD------------------------------------------------------------------------------
               M____H____                                                                              
                CCCCEECCC                                                                              
                ZZddddeZZ

Scripts     (PyMol,     Modeller,     ...)

PyMol 
	Download
Modeller 
	
If you want to use this structure as a template for modelisation with Modeller, you can use the following sequence to build the alignment file "*.ali".

To avoid the classical Modeller error :

			'Number of residues in the alignment and pdb files are different'(see FAQ n°17),

the missing residues, non-classical amino-acids, and sequence gaps have been replaced by a "X" symbol.
XKRHRXVLRD 
1) align this sequence with your sequence to modelised;
2) replace the "X" by "-" in the obtained alignement, then build your "*.ali" file;
3) use Modeller to make your models;
4) And finally use the following information found in the PDB to add this PTM in your models (see FAQ n°8 & 9 and this archive of Modeller usage).
			

			

			
				

MODRES 3F9X MLY G   20  LYS  N-DIMETHYL-LYSINE                                  
SHEET    4   K 4 MLY G  20  VAL G  21  1  O  MLY G  20   N  TYR C 274           
LINK         C   ARG G  19                 N   MLY G  20     1555   1555  1.33  
LINK         C   MLY G  20                 N   VAL G  21     1555   1555  1.34  
HETATM 5322  N   MLY G  20      -3.153  12.228 -32.196  1.00 27.14           N  
ANISOU 5322  N   MLY G  20     3402   3329   3581    271     35   -155       N  
HETATM 5323  CA  MLY G  20      -3.479  12.830 -30.901  1.00 25.37           C  
ANISOU 5323  CA  MLY G  20     3174   3139   3328    232    -73   -178       C  
HETATM 5324  CB  MLY G  20      -4.250  14.130 -31.088  1.00 25.37           C  
ANISOU 5324  CB  MLY G  20     3122   3137   3382    216    -93   -120       C  
HETATM 5325  CG  MLY G  20      -3.584  15.109 -32.062  1.00 25.30           C  
ANISOU 5325  CG  MLY G  20     3175   2979   3460    294    -34     14       C  
HETATM 5326  CD  MLY G  20      -4.611  16.105 -32.623  1.00 25.85           C  
ANISOU 5326  CD  MLY G  20     3240   2959   3622    210    119     37       C  
HETATM 5327  CE  MLY G  20      -4.130  16.929 -33.824  1.00 27.42           C  
ANISOU 5327  CE  MLY G  20     3426   3397   3596    134    -23   -125       C  
HETATM 5328  NZ  MLY G  20      -3.795  16.103 -34.996  1.00 26.51           N  
ANISOU 5328  NZ  MLY G  20     3142   3157   3772   -138   -196   -444       N  
HETATM 5329  CH1 MLY G  20      -4.896  15.181 -35.383  1.00 25.40           C  
ANISOU 5329  CH1 MLY G  20     2900   3218   3532   -482   -631   -652       C  
HETATM 5330  CH2 MLY G  20      -3.518  16.965 -36.154  1.00 27.13           C  
ANISOU 5330  CH2 MLY G  20     3369   3142   3798     -7     73   -379       C  
HETATM 5331  C   MLY G  20      -4.239  11.820 -30.097  1.00 23.38           C  
ANISOU 5331  C   MLY G  20     2883   2939   3063    315   -130   -261       C  
HETATM 5332  O   MLY G  20      -5.065  11.078 -30.659  1.00 21.97           O  
ANISOU 5332  O   MLY G  20     2576   2875   2897    459   -350   -559       O