Sequence     &     Structural     Information
	
PTM
PTM Formylation
Sequence Information
Uniprot AC P61829
Organism YEAST (Baker's yeast)
Modified Amino Acid M(Met) 1
Annotation dbPTM details N-formylmethionine.
Structure Information
PDB ID Code 4F4S
Model -
Chain B
Position 1
SCOP Class None
MODRES N-FORMYLMETHIONINE
Related PTM
Found in same PDB Chain -
Found in same Uniprot AC
Sort by: PDB ID Code (idpdb-chain_aa) Sequence position (aa_idpdb-chain)
Gallery (?)
Slide 1
Slide 2
Example Frame



Note: As the images production is automatic, the focus on the PTM site could not be the most optimized.
      In these cases it is recommended to use the PyMOL script to visualize the PTM site in 3D.
Alignment
(?)

	           Numerotation

	(?)       Uniprot Sequence
	(?)                Clustal
	(?)           PDB Sequence
	(?)        PDB Information
	(?)          DSSP Sequence
	(?) Protein Block Sequence
	
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MQLVLAAKYIGAGISTIGLLGAGIGIAIVFAALINGVSRNPSIKDTVFPMAILGFALSEATGLFCLMVSFLLLFGV
 ***************************************************************************
XQLVLAAKYIGAGISTIGLLGAGIGIAIVFAALINGVSRNPSIKDTVFPMAILGFALSEATGLFCLMVSFLLLFGV
H__________________________________________________________________________M
CHHHHHHHHHHHHHTTTTHHHHHHHHHHHHHHHHHHHHHCGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC 
ZZmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmlmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmZZ 

Scripts     (PyMol,     Modeller,     ...)

PyMol
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Modeller
	
If you want to use this structure as a template for modelisation with Modeller, you can use the following sequence to build the alignment file "*.ali".

To avoid the classical Modeller error :

			'Number of residues in the alignment and pdb files are different'(see FAQ n°17),

the missing residues, non-classical amino-acids, and sequence gaps have been replaced by a "X" symbol.
			
XQLVLAAKYIGAGISTIGLLGAGIGIAIVFAALINGVSRNPSIKDTVFPMAILGFALSEATGLFCLMVSFLLLFGX
1) align this sequence with your sequence to modelised;
2) replace the "X" by "-" in the obtained alignement, then build your "*.ali" file;
3) use Modeller to make your models;
4) And finally use the following information found in the PDB to add this PTM in your models (see FAQ n°8 & 9 and this archive of Modeller usage).
			
REMARK 470 FME B 1 CG SD CE
REMARK 500 FME B 1 O - C - N ANGL. DEV. = -12.6 DEGREES
REMARK 500 FME B 1 -16.59
MODRES 4F4S FME B 1 MET N-FORMYLMETHIONINE
LINK C FME B 1 N GLN B 2 1555 1555 1.42
HETATM 547 N FME B 1 37.669 15.376 64.393 1.00 51.24 N
ANISOU 547 N FME B 1 8086 7476 3904 485 708 -603 N
HETATM 548 CN FME B 1 36.867 14.320 64.198 1.00 49.45 C
ANISOU 548 CN FME B 1 7393 7513 3882 683 685 -431 C
HETATM 549 O1 FME B 1 36.777 13.784 63.096 1.00 48.07 O
ANISOU 549 O1 FME B 1 6958 7385 3920 667 629 -356 O
HETATM 550 CA FME B 1 38.153 16.175 63.270 1.00 50.84 C
ANISOU 550 CA FME B 1 8323 7156 3835 345 721 -694 C
HETATM 551 CB FME B 1 38.515 17.583 63.735 1.00 54.59 C
ANISOU 551 CB FME B 1 9562 7263 3915 195 833 -868 C
HETATM 552 C FME B 1 39.334 15.531 62.599 1.00 48.59 C
ANISOU 552 C FME B 1 7699 7023 3738 -44 535 -709 C
HETATM 553 O FME B 1 39.378 15.533 61.348 1.00 47.10 O
ANISOU 553 O FME B 1 7428 6761 3707 -36 519 -686 O