Sequence     &     Structural     Information
	
PTM
PTM N-linked Glycosylation
Sequence Information
Uniprot AC P00533
Organism HUMAN (Human)
Modified Amino Acid N(Asn) 361
Annotation dbPTM details N-linked (GlcNAc...).
N-linked (Glc...)
Structure Information
PDB ID Code 4KRM
Model -
Chain C
Position 337
SCOP Class None
MODRES GLYCOSYLATION SITE
Related PTM
Found in same PDB Chain 328 420
Found in same Uniprot AC
Sort by: PDB ID Code (idpdb-chain_aa) Sequence position (aa_idpdb-chain)
Gallery (?)
Slide 1
Slide 2
Example Frame



Note: As the images production is automatic, the focus on the PTM site could not be the most optimized.
      In these cases it is recommended to use the PyMOL script to visualize the PTM site in 3D.
Alignment
(?)

	           Numerotation

	(?)       Uniprot Sequence
	(?)                Clustal
	(?)           PDB Sequence
	(?)        PDB Information
	(?)          DSSP Sequence
	(?) Protein Block Sequence
	
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MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA
                                                                                                                                                                                                                                                                                                                             :**             :************************************************************************************************************************************************************************************************************ :  .                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LEE-------------KKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKHHHHHH------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
                                                                                                                                                                                                                                                                                                                             SSS             S_________________________________________________________________________________________________________________________________________________________________________________________________MMMMMMMMMMMMMMMMM                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                             CEE             EEEEECBTSGGGTTCSSCCTTGGGGGTTCSEEESBEEECTHHHHCBGGGTBCCCCGGGGGGGGGCCEESSCEEESCCCTTCCSCGGGTTCCEECCSSCBTTTEEEEEESCCCSCCCCTTCCEECSSEEEEECCTTCCSGGGCCGGGTBCSSSCCEEEESSSCHHHHHHTTCSCCTTBCTTCBSSSSGGGBSSBC                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                             ZZd             dddddehiajllmpcbdcfklmmmmgoiabdcddddddfklmnopfknopacddfklmmmmmmpmbdfbdcddfbdcfklcbffklmmbfbdgklacehhlafbdcehiabfbfolakbcbdfbacdddehilkbghklmpcfklmpmbfklccddehiacfklmmmmmcdcdfkbcfkbpabjbfklpmbdZZ                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

Scripts     (PyMol,     Modeller,     ...)

PyMol
	Download
Modeller
	
If you want to use this structure as a template for modelisation with Modeller, you can use the following sequence to build the alignment file "*.ali".

To avoid the classical Modeller error :

			'Number of residues in the alignment and pdb files are different'(see FAQ n°17),

the missing residues, non-classical amino-acids, and sequence gaps have been replaced by a "X" symbol.
			
LEEKKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRXXXXXXXXXXXXXXXXX
1) align this sequence with your sequence to modelised;
2) replace the "X" by "-" in the obtained alignement, then build your "*.ali" file;
3) use Modeller to make your models;
4) And finally use the following information found in the PDB to add this PTM in your models (see FAQ n°8 & 9 and this archive of Modeller usage).
			
REMARK 500 ND2 ASN C 337 O5 NAG C 605 1.82
MODRES 4KRM ASN C 337 ASN GLYCOSYLATION SITE
LINK ND2 ASN C 337 C1 NAG C 605 1555 1555 1.55
ATOM 5082 N ASN C 337 12.055 11.578 0.526 1.00 57.67 N
ATOM 5083 CA ASN C 337 12.668 12.513 1.489 1.00 55.75 C
ATOM 5084 C ASN C 337 14.052 13.002 1.067 1.00 53.75 C
ATOM 5085 O ASN C 337 14.778 13.574 1.880 1.00 57.11 O
ATOM 5086 CB ASN C 337 12.780 11.834 2.854 1.00 58.94 C
ATOM 5087 CG ASN C 337 11.462 11.808 3.602 1.00 73.28 C
ATOM 5088 OD1 ASN C 337 10.438 12.265 3.095 1.00 77.27 O
ATOM 5089 ND2 ASN C 337 11.481 11.270 4.817 1.00 80.61 N
ATOM 5090 H ASN C 337 11.387 11.868 0.051 1.00 69.20 H
ATOM 5091 HA ASN C 337 12.131 13.330 1.648 1.00 66.90 H
ATOM 5092 HB2 ASN C 337 13.071 10.917 2.727 1.00 70.73 H
ATOM 5093 HB3 ASN C 337 13.234 12.665 3.482 1.00 70.73 H
ATOM 5094 HD21 ASN C 337 12.079 10.958 5.410 1.00 96.73 H
HETATM28356 C1 NAG C 605 9.994 11.044 5.185 1.00 84.87 C
HETATM28357 C2 NAG C 605 9.875 10.133 6.397 1.00 83.15 C
HETATM28358 C3 NAG C 605 8.719 10.655 7.256 1.00 89.76 C
HETATM28359 C4 NAG C 605 9.035 12.105 7.633 1.00 96.86 C
HETATM28360 C5 NAG C 605 9.168 12.923 6.348 1.00 87.99 C
HETATM28361 C6 NAG C 605 9.590 14.337 6.705 1.00 84.39 C
HETATM28362 C7 NAG C 605 10.443 8.093 5.213 1.00 83.60 C
HETATM28363 C8 NAG C 605 10.025 6.708 4.818 1.00 79.79 C
HETATM28364 N2 NAG C 605 9.580 8.784 5.953 1.00 84.28 N
HETATM28365 O3 NAG C 605 8.677 9.902 8.443 1.00 90.22 O
HETATM28366 O4 NAG C 605 7.937 12.618 8.348 1.00103.32 O
HETATM28367 O5 NAG C 605 10.263 12.358 5.623 1.00 87.28 O
HETATM28368 O6 NAG C 605 8.524 14.863 7.499 1.00 84.74 O
HETATM28369 O7 NAG C 605 11.532 8.541 4.855 1.00 80.49 O
HETATM28370 H1 NAG C 605 9.273 11.012 4.497 1.00101.85 H
HETATM28371 H2 NAG C 605 10.894 10.080 6.814 1.00 99.78 H
HETATM28372 H3 NAG C 605 7.797 10.418 6.699 1.00107.71 H
HETATM28373 H4 NAG C 605 9.870 12.044 8.347 1.00116.23 H
HETATM28374 H5 NAG C 605 8.116 13.045 6.011 1.00105.59 H
HETATM28375 H61 NAG C 605 10.030 14.757 5.779 1.00101.27 H
HETATM28376 H62 NAG C 605 10.440 14.247 7.382 1.00101.27 H
HETATM28377 H81 NAG C 605 10.496 6.655 3.743 1.00 95.74 H
HETATM28378 H82 NAG C 605 8.970 6.794 4.555 1.00 95.74 H
HETATM28379 H83 NAG C 605 9.927 6.218 5.882 1.00 95.74 H
HETATM28380 HN2 NAG C 605 8.702 8.361 6.218 1.00101.14 H
HETATM28381 HO3 NAG C 605 9.524 9.424 8.556 1.00108.26 H
HETATM28382 HO6 NAG C 605 8.367 15.799 7.258 1.00101.69 H