Receptor expression-enhancing protein 5 (REEP5)
The protein contains 189 amino acids for an estimated molecular weight of 21493 Da.
May promote functional cell surface expression of olfactory receptors. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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Biological Process
Endoplasmic reticulum organization
Endoplasmic reticulum tubular network organization
Regulation of intracellular transport
Cellular Component
Endoplasmic reticulum
Endoplasmic reticulum tubular network
Extracellular exosome
Integral component of membrane
Membrane
Molecular Function
The reference OMIM entry for this protein is 125265
Receptor expression-enhancing protein 5; reep5
Deleted in polyposis 1; dp1
Tb2
D5s346
Yop1, s. cerevisiae, homolog of; yop1
Chromosome 5 open reading frame 18; c5orf18
CLONING
Through study of submicroscopic deletions in 2 unrelated patients with familial adenomatous polyposis coli (FAP; 175100), Joslyn et al. (1991) found 3 genes in a 100-kb deleted segment on chromosome 5. MCC (159350), a previous candidate gene, was shown to be located outside the deleted region. One of the new genes contained a sequence identical to SRP19 (182175). The second gene, provisionally designated DP1, was transcribed in the same orientation as MCC. Two other cDNAs, DP2 and DP3, were found to overlap, forming a single gene, DP2.5, which was transcribed in the same orientation as SRP19. The DP2.5 gene was shown by Groden et al. (1991) to be the polyposis gene, as indicated in 611731. Transport of G protein-coupled receptors (GPCRs) to the cell surface membrane is critical for receptor-ligand recognition. However, mammalian GPCR odorant receptors (ORs), when heterologously expressed in cells, are poorly expressed on the cell surface. By screening for genes that induced cell surface expression of ORs expressed in human embryonic kidney cells, Saito et al. (2004) identified mouse and human REEP1 (609139). They searched databases for homologs of REEP1 and identified several other REEP genes, including DP1, which they called REEP5. In situ hybridization of mouse olfactory epithelium revealed that, unlike Reep1, Reep5 was not expressed in olfactory neurons.GENE FUNCTION
Using an in vitro system to identify Xenopus membrane proteins involved in endoplasmic reticulum (ER) network formation, followed by localization, overexpression, and deletion experiments in mammalian and yeast cells, Voeltz et al. (2006) identified the reticulons, particularly Rtn4a/NogoA (604475), and the reticulon-interacting protein DP1/Yop1 as the major components shaping the tubular ER.MAPPING
Joslyn et al. (1991) identified the DP1 gene in a 100-kb segment on chromosome 5 deleted in 2 patients with APC. ... More on the omim web siteMay 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 125265 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed