40S ribosomal protein S15 (RPS15)

The protein contains 145 amino acids for an estimated molecular weight of 17040 Da.

 

No function (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 100%
Model score: 100

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The reference OMIM entry for this protein is 180535

Ribosomal protein s15; rps15
Rat insulinoma gene homolog; rig

CLONING

The gene called Rig (rat insulinoma gene) was first isolated from a cDNA library of rat insulinomas. Its cognate gene has been found to be activated in various human tumors such as insulinomas, esophageal cancers, and colon cancers. Inoue et al. (1987) isolated a human insulinoma cDNA encoding the human homolog of Rig. Structural analysis indicated that the predicted 145-amino acid RIG protein may be a DNA-binding protein. Because of CpG islands in the 5-prime region and regions with a high GC content in the human RIG gene and because of the wide expression of RIG in tissues and cells, Shiga et al. (1990) suggested that RIG may belong to the class of 'housekeeping' genes, whose products are necessary for the growth of all cell types. Kitagawa et al. (1991) showed that the immunoreactivity to a monoclonal antibody against the deduced Rig protein and the translation product of Rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of Rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. The authors concluded that the product of the Rig gene is ribosomal protein S15.

GENE STRUCTURE

Shiga et al. (1990) isolated the genomic sequence of human RIG from a genomic DNA library constructed from a human esophageal carcinoma and determined its complete nucleotide sequence. The gene is composed of about 3,000 nucleotides and divided into 4 exons. The transcription initiation site was located -46 bp upstream from the first ATG codon. The human genome contains at least 6 copies of RIG pseudogenes, 4 of which have the characteristics of processed pseudogenes.

MAPPING

By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi et al. (1998) mapped the human RPS15 gene to chromosome 19p. ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 16, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 26, 2017: Protein entry updated
Automatic update: model status changed

March 15, 2016: Protein entry updated
Automatic update: OMIM entry 180535 was added.

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed