60S ribosomal protein L10a (RPL10A)
The protein contains 217 amino acids for an estimated molecular weight of 24831 Da.
Component of the large ribosomal subunit. (updated: Nov. 22, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs17838763 |
Biological Process
Anatomical structure morphogenesis
Cellular protein metabolic process
Cytoplasmic translation
Gene expression
Maturation of LSU-rRNA
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Response to ethanol
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational elongation
Translational initiation
Translational termination
Viral life cycle
Viral process
Viral transcription
The reference OMIM entry for this protein is 615660
Ribosomal protein l10a; rpl10a
Csa19
CLONING
Using subtractive hybridization, Fisicaro et al. (1995) identified Rpl10a, which they called CsA19, among cDNA clones whose expression was downregulated in mouse thymus after exposure to the immunosuppressive drug cyclosporin A (CsA). By screening a human mesangial cell cDNA library with the mouse cDNA, they obtained human CsA19. The mouse and human CsA19 proteins both contain 217 amino acids and share 99% identity. CsA19 has a potential nuclear localization signal, a lysine-rich region, and a tyrosine kinase phosphorylation site. Southern blot analysis showed conservation of CsA19 in mammals, chicken, and yeast. Northern blot analysis of mouse tissues revealed highest CsA19 expression in lymph node, followed by spleen, thymus, lung, and bone marrow. Immunoblot analysis showed expression of an approximately 30-kD protein in mouse lymph node extracts.MAPPING
Gross (2014) mapped the RPL10A gene to 6p21.31 based on an alignment of the RPL10A sequence (GenBank GENBANK AB082926) with the genomic sequence (GRCh37). There are multiple RPL10A pseudogenes located throughout the genome. ... More on the omim web site
Feb. 5, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 615660 was added.