PDZK1-interacting protein 1 (PDZK1IP1)
The protein contains 114 amino acids for an estimated molecular weight of 12227 Da.
May play an important role in tumor biology. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Molecular Function
The reference OMIM entry for this protein is 607178
Membrane-associated protein 17
Map17
Dd96
CLONING
Using the differential display technique, Kocher et al. (1996) identified a epithelial-specific gene, originally designated DD96, which was markedly upregulated in various human carcinomas originating from the kidney, colon, lung, and breast, as compared with normal epithelial cell populations. By Northern blot analysis and in situ hybridization, Kocher et al. (1995) found that, in normal tissues, MAP17 is expressed at significant levels only in a single epithelial cell population, the proximal tubular epithelial cells of the kidney. By Western blot analysis, Kocher et al. (1996) determined that the DD96 protein product is a 17-kD membrane-associated protein, which they redesignated MAP17. By immunofluorescence studies, they localized MAP17 to the apical brush border membrane. In permeabilized cultured cell lines, MAP17 was associated with cell-cell membrane contact areas, indicating that MAP17 is an intracellular protein associated with the cytoplasmic aspect of the plasma membrane. Transfection of a full-length wildtype MAP17 cDNA clone into a colon carcinoma cell line markedly decreased cell proliferation in vitro and tumor growth in vivo.GENE FUNCTION
Using the yeast 2-hybrid system, Kocher et al. (1998) identified PDZK1 (603831) as a protein interacting with MAP17. By in situ hybridization, they showed that both proteins are upregulated in human carcinomas arising in kidney, lung, colon, and breast.MAPPING
Gottgens et al. (2000, 2001) demonstrated that the MAP17 gene lies downstream of the stem cell leukemia gene SCL (187040) in human, mouse, and chicken genomes; Gottgens et al. (2002) showed that it is located in a comparable position in zebrafish as well. Human MAP17 maps to chromosome 1p33. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for PDZK1IP1
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607178 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed