Elongin-C (TCEB1)
The protein contains 112 amino acids for an estimated molecular weight of 12473 Da.
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed:7821821). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells (By similarity).', 'The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. (updated: Dec. 20, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cellular response to hypoxia
Gene expression
Interleukin-7-mediated signaling pathway
Positive regulation of transcription elongation from RNA polymerase II promoter
Positive regulation of viral transcription
Post-translational protein modification
Protein ubiquitination
Protein ubiquitination involved in ubiquitin-dependent protein catabolic process
Regulation of transcription by RNA polymerase II
Regulation of transcription from RNA polymerase II promoter in response to hypoxia
Transcription by RNA polymerase II
Transcription elongation from RNA polymerase II promoter
Ubiquitin-dependent protein catabolic process
Viral process
Cellular Component
Cul2-RING ubiquitin ligase complex
Cytosol
Elongin complex
Nucleoplasm
VCB complex
The reference OMIM entry for this protein is 600788
Transcription elongation factor b, polypeptide 1; tceb1
Elongin c; eloc
Elongin, 15-kd subunit
CLONING
The elongin (SIII) complex is a heterotrimer composed of 3 subunits, called A, B, and C, of 110, 18, and 15 kD, respectively. Aso et al. (1995) demonstrated that the 110-kD polypeptide designated elongin A (600786) functions as the transcriptionally active component of the SIII complex, whereas elongins B (600787) and C (TCEB1) are regulatory subunits. Garrett et al. (1994) isolated and sequenced the human TCEB1 gene (which they had referred to as p15).GENE FUNCTION
Duan et al. (1995) and Kibel et al. (1995) reported results suggesting that the tumor suppression activity of the von Hippel-Lindau tumor suppressor gene product (608537) is a function of its ability to bind to elongin B (TCEB2) and C (TCEB1) and thereby inhibit transcription elongation.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the TCEB1 gene to chromosome 8 (TMAP stSG4816). - Pseudogene A gene designated TCEB1L because of its high sequence similarity to TCEB1 was later found to be the same as OCP2 (SKP1A; 601434), which maps to chromosome 5. Demetrick et al. (1996) mapped the SKP1A gene to 7q11.2 by fluorescence in situ hybridization, but this was later determined to be a pseudogene of TCEB1 (TCEB1P). ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 600788 was added.