Protein phosphatase 1 regulatory subunit 7 (PPP1R7)
The protein contains 360 amino acids for an estimated molecular weight of 41564 Da.
Regulatory subunit of protein phosphatase 1. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 602877
Protein phosphatase 1, regulatory subunit 7; ppp1r7
Sds22, s. pombe, homolog of
CLONING
Type 1 serine/threonine phosphatases consist of a catalytic subunit and one or more regulatory subunits. The noncatalytic subunits not only determine the activity and substrate specificity of the holoenzyme, but also target the phosphatase to a particular cellular location. S. pombe sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis. By sequence analysis of a human brain expressed sequence tag (EST) that is homologous to sds22, and by 5-prime RACE using oligonucleotides based on this EST, Renouf et al. (1995) determined the complete cDNA sequence of a human homolog of sds22, termed PPP1R7. The predicted 360-amino acid PPP1R7 protein contains 11 leucine-rich repeats, which may participate in protein-protein interactions, and multiple putative phosphorylation sites. The amino acid sequences of PPP1R7 and sds22 are 46% identical. Western blot analysis using antibodies against human PPP1R7 detected a 44-kD protein in both the cytoplasm and nucleus of rat liver cells. Northern blot analysis showed a major 1.4-kb transcript in all human tissues examined.MAPPING
By PCR analysis of YACs and somatic cell hybrid DNAs using sequence tagged sites derived from the 3-prime untranslated region of PPP1R7, Berry et al. (1995) mapped the PPP1R7 gene to 2q37.3. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 602877 was added.