DDB1- and CUL4-associated factor 6 (DCAF6)
The protein contains 860 amino acids for an estimated molecular weight of 96292 Da.
Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11558511 |
Biological Process
Positive regulation of transcription by RNA polymerase II
Post-translational protein modification
Protein ubiquitination
Cellular Component
Cul4-RING E3 ubiquitin ligase complex
Cytoplasm
Cytosol
Focal adhesion
Nucleoplasm
Nucleus
The reference OMIM entry for this protein is 610494
Ddb1- and cul4-associated factor 6; dcaf6
Iq motif- and wd repeats-containing 1; iqwd1
Nuclear receptor interaction protein; nrip
Hom-tes-88/94/95
CLONING
By subtractive hybridization to enrich for transcripts highly expressed in testis, followed by serologic expression screening with antibodies from a seminoma patient, Tureci et al. (2002) isolated IQWD1, which they designated HOM-TES-88/94/95. RT-PCR detected ubiquitous expression. Using androgen receptor (AR; 313700) as bait in a yeast 2-hybrid screen of a HeLa cell cDNA library, Tsai et al. (2005) cloned IQWD1, which they called NRIP. The deduced 860-amino acid protein has a calculated molecular mass of 96 kD. It contains 7 WD40 repeats, 5 of which are N-terminal and 2 of which are C-terminal following a nuclear localization signal. Tsai et al. (2005) also identified the mouse Nrip gene, which shares 86.3% homology with human NRIP. Northern blot analysis detected highest expression in skeletal muscle and testis, and weaker expression in heart, prostate, and adrenal gland. NRIP was also detected in all cancer cell lines examined. Fluorescence-tagged NRIP was expressed in the nuclei of transfected human embryonic kidney cells.GENE FUNCTION
Using in vitro pull-down assays of in vitro translated proteins and immunoprecipitation analysis of exogenously expressed proteins, Tsai et al. (2005) demonstrated that NRIP interacted directly with AR and glucocorticoid receptor (GCCR; 138040) in the presence of ligand. NRIP also enhanced AR- and GCCR-mediated transcriptional activity in a reporter gene assay in the presence of AR or GCCR ligands. Downregulation of endogenous NRIP by siRNA reduced the growth rate of a human prostate carcinoma cell line in the absence of ligand. Tsai et al. (2005) concluded that NRIP functions as a ligand-dependent coactivator of nuclear receptors and enhances their transcriptional activity.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the DCAF6 gene to chromosome 1 (TMAP RH64705). ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610494 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed