Tubulin-specific chaperone cofactor E-like protein (TBCEL)
The protein contains 424 amino acids for an estimated molecular weight of 48195 Da.
Acts as a regulator of tubulin stability. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
The reference OMIM entry for this protein is 610451
Leucine-rich repeat-containing protein 35; lrrc35
E-like protein; el
CLONING
By searching human EST databases with C. elegans Lrrc35, followed by PCR of a human testis cDNA library, Bartolini et al. (2005) cloned LRRC35, which they named E (TBCE; 604934)-like. The deduced 425-amino acid protein shares 23% amino acid identity with TBCE and contains a leucine-rich repeat followed by a ubiquitin-like motif. Northern blot analysis detected a 6.0-kb transcript with high expression in testis and much lower expression in heart, liver, spleen, and muscle.GENE FUNCTION
Using in vitro folding assays, Bartolini et al. (2005) showed that, in contrast to TBCE, LRRC35 did not play a role in tubulin heterodimer assembly and had no effect on the tubulin (see 602529) GTPase activity of cofactor C (TBCC; 602971) and cofactor D (TBCD; 604649). Overexpression of LRRC35 caused microtubule destruction by disrupting tubulin heterodimers and targeting tubulin to the proteasome for degradation. Using siRNA, they showed that cells depleted of LRRC35 retained stable microtubules, mostly concentrated in the perinuclear region, and displayed a clustering of endoplasmic reticulum, Golgi, and lysosomal organelle membranes in a condensed region of the cytoplasm. Bartolini et al. (2005) concluded that LRRC35 regulation of microtubule network stability affects vesicle trafficking mediated by microtubule-dependent molecular motors.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the LRRC35 gene to chromosome 11 (TMAP SHGC-57160). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610451 was added.