Suprabasin (SBSN)
The protein contains 590 amino acids for an estimated molecular weight of 60541 Da.
No function (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs10775583 |
No binding partner found
Biological Process
Molecular Function
The reference OMIM entry for this protein is 609969
Suprabasin
Sbsn
CLONING
By subtractive hybridization to identify genes expressed in differentiated mouse keratinocytes, Park et al. (2002) cloned Sbsn. The deduced 700-amino acid protein has a calculated molecular mass of 72 kD. Sbsn contains an N-terminal transmembrane domain, a central domain with glycine-, alanine-, and histidine-rich short tandem repeats, and several phosphorylation and myristoylation sites. An alternatively spliced transcript encodes a protein lacking the central repetitive region. By RT-PCR of skin RNA, Park et al. (2002) cloned a partial human SBSN cDNA. Northern blot analysis of human tissues detected 2.2- and 1.1-kb SBSN transcripts in skin, esophagus, uterus, thymus, and a keratinocyte cell line. Northern blot analysis detected 2 Sbsn transcripts at embryonic day 15.5 in mice, coinciding with epidermal stratification. In situ hybridization of embryonic and adult mice showed Sbsn expression in the suprabasal layers of the epithelia in tongue, stomach, and epidermis. Northern and RNA dot blot analyses of adult mouse tissues revealed expression in uterus and thyroid, but not in heart, brain, spleen, lung, liver, smooth muscle, and kidney. Fluorescence-tagged Sbsn showed a punctate cytoplasmic distribution in transfected mouse keratinocytes that did not overlap with lysosomal or mitochondrial markers.GENE FUNCTION
Park et al. (2002) found that expression of Sbsn in primary mouse epidermal keratinocytes was induced by calcium and phorbol esters and inhibited by a PKC (see PRKCA; 176960) inhibitor. Monomeric recombinant Sbsn was cross-linked into a high molecular mass form by the transglutaminases Tgm2 (190196) and Tgm3 (600238). Park et al. (2002) concluded that SBSN may have a role in epidermal differentiation.GENE STRUCTURE
Park et al. (2002) determined that the mouse Sbsn gene contains 4 exons.MAPPING
By genomic sequence analysis, Park et al. (2002) mapped the SBSN gene to chromosome 19q13.1. They mapped the mouse Sbsn gene to a region of chromosome 7B2-B3 that shares homology of synteny with human chromosome 19q13.1. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for SBSN
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 609969 was added.