ATP-binding cassette sub-family F member 1 (ABCF1)
The protein contains 845 amino acids for an estimated molecular weight of 95926 Da.
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs6902544 |
Biological Process
Inflammatory response
Positive regulation of translation
Translation
Translational initiation
Transmembrane transport
Cellular Component
Cytoplasm
Cytosol
Membrane
Nuclear envelope
Nucleoplasm
Polysomal ribosome
Ribosome
The reference OMIM entry for this protein is 603429
Atp-binding cassette, subfamily f, member 1; abcf1
Atp-binding cassette 50; abc50
CLONING
The family of ATP-binding cassette (ABC) proteins (e.g., 601691) is extensive and is responsible for the transport of a variety of molecules across biologic membranes. Using differential display PCR to identify genes that are modulated by TNF-alpha (191160) in synoviocytes, Richard et al. (1998) isolated a cDNA encoding a novel member of the ABC family, named ABC50. The deduced 807-amino acid ABC50 protein has 2 ABC transporter family signature motifs, 2 ATP/GTP-binding site A motifs, and 3 nuclear localization patterns. The authors found that ABC50 also contains a signature sequence that is characteristic of yeast cluster IV ABC proteins. Unlike most previously reported family members, ABC50 does not contain a transmembrane domain. Northern blot analysis demonstrated ubiquitous expression of a 3.7-kb ABC50 transcript in human tissues. TNF-alpha stimulation increased the expression of the ABC50 mRNA in cultured synoviocytes.GENE STRUCTURE
Berezikov et al. (2007) determined that intron 13 of the ABCF1 gene contains the microRNA MIRN877 (611619).MAPPING
Richard et al. (1998) used fluorescence in situ hybridization to map the human ABC50 gene to chromosome 6p21.33. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603429 was added.