Osteoclast-stimulating factor 1 (OSTF1)
The protein contains 214 amino acids for an estimated molecular weight of 23787 Da.
Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs2295862 | |
| dbSNP:rs17850197 |
The reference OMIM entry for this protein is 610180
Osteoclast-stimulating factor 1; ostf1
Osf
Sh3p2
DESCRIPTION
Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998).CLONING
Using an expression cloning approach, followed by 5-prime RACE, Reddy et al. (1998) cloned human OSTF1, which they designated OSF, from an osteoclast-like multinucleated cell (OST-like MNC) expression library. The deduced 214-amino acid OSTF1 protein has a predicted molecular mass of 28 kD and shares 95% sequence identity with the mouse homolog. It contains an N-terminal proline-rich region and a potential N-glycosylation site, but lacks a signal sequence. OSTF1 has ankyrin repeats and an Src homology-3 (SH3) domain that is homologous to the SH3 domain of GRB2 (108355) and FYN (137025). Northern blot analysis detected ubiquitous expression of a 1.3-kb transcript in multiple human tissues. In situ hybridization demonstrated OSTF1 expression in giant cells isolated from osteosarcoma and in osteoclast-like cells in long-term bone marrow cultures.GENE FUNCTION
Reddy et al. (1998) demonstrated that conditioned media from HEK293 cells transfected with OSTF1 cDNA stimulated MNC formation in murine and human bone marrow cultures. OSTF1 also increased bone resorption in the transfected cells. Both OSTF1 activities occurred in the presence or absence of 1,25-dihydroxy vitamin D3. Reddy et al. (1998) concluded that OSTF1 recruits osteoclasts and activates them to resorb bone. Using Western blot analysis of OSTF1 cellular distribution, Reddy et al. (1998) found that OSTF1 was not secreted into the culture media. They suggested that transient expression of OSTF1 cDNA in HEK293 cells results in the release of other factors that enhance osteoclast formation. In vitro affinity binding of recombinant OSTF1 with a GST-c-Src/SH3-SH2 fusion protein suggested that the effect of OSTF1 stimulation on osteoclast formation may occur through indirect signaling mediated by Src (190090) or other Src-related proteins.MAPPING
By FISH, Schaub et al. (2000) mapped the OSTF1 gene to chromosome 12q24.1-q24.2. However, the International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 9 (TMAP SGC35040). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610180 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed